FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76419
Sequences flagged as poor quality0
Sequence length301
%GC55

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC18052.3619780421099463No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG17142.242897708685013No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG16672.181394679333674No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG16492.1578403276672034No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC16042.0989544485010274No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG15782.0649314960939034No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA15362.0099713422054726No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC14921.9523940381318783No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG14801.936691137020898No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA13331.7443305984113897No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA13131.718159096559756No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG12611.6501131917455083No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG12491.634410290634528No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC12441.6278674151716195No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG11971.5663643858202803No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG11311.479998429709889No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA11221.4682212538766537No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC11151.459061228228582No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG11091.4512097776730917No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA9231.2078148104528978No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA9001.177717583323519No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG8261.080883026472474No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC8131.0638715502689122No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA7901.0337743231395333No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA7450.9748884439733574No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG7060.9238540153626715No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA6890.9016082387887829No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA6300.8244023083264632No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA5500.7197163009199283No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC5330.6974705243460396No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG4470.5849330663840144No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG4250.5561444143472173No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA4160.5443672385139822No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3980.5208128868475117No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3920.5129614362920216No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA3870.5064185608291132No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3610.4723956084219893No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCG3600.4710870333294076No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC3560.46585273295908086No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCG3530.46192700768133577No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG3500.4580012824035908No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG3430.44884125675551895No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3330.4357555058297021No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3250.42528690508904854No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG3080.4030411285151599No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3060.40042397832999643No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCG3050.3991154032374148No Hit
CCTANGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC3040.39780682814483315No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3030.3964982530522514No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3020.3951896779596697No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA3020.3951896779596697No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3010.39388110286708805No Hit
CCTANGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC3010.39388110286708805No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2940.3847210772190162No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2840.37163532629319934No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2800.3664010259228726No Hit
CCTANGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA2790.3650924508302909No Hit
CCTANGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2780.3637838757377092No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2780.3637838757377092No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2770.36247530064512756No Hit
CCTANGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA2760.3611667255525458No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2720.35593242518221907No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2600.34022952407123885No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCG2480.3245266229602586No Hit
CCTANGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA2440.31929232258993184No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2390.3127494471270234No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA2340.3062065716641149No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAC2330.30489799657153327No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2300.3009722712937882No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2220.2905036705531347No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2180.28526937018280796No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2180.28526937018280796No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2150.28134364490506286No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCG2150.28134364490506286No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAC2010.26302359360891925No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2000.26171501851633755No Hit
CCTANGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1930.25255499286826577No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAC1920.25124641777568407No Hit
CCTANGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1870.2447035423127756No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1870.2447035423127756No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCG1780.23292636647954043No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1780.23292636647954043No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1770.23161779138695873No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1740.22769206610921366No Hit
CCTANGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1740.22769206610921366No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1730.22638349101663202No Hit
CCTANGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1620.2119891649982334No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAC1620.2119891649982334No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1480.1936691137020898No Hit
CCTANGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1450.18974338842434474No Hit
CCTANGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1440.18843481333176304No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAC1430.18712623823918137No Hit
CCTANGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1360.17796621259110953No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT1300.17011476203561943No Hit
CCTANGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1280.16749761185045606No Hit
CCTANGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1230.1609547363875476No Hit
CCTANGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1230.1609547363875476No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT1210.15833758620238422No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT1190.15572043601722085No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT1140.14917756055431242No Hit
CCTANGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1140.14917756055431242No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1040.13609180962849554No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT950.12431463379526035No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT950.12431463379526035No Hit
CCTANGGGTGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG930.12169748361009697No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT920.12038890851751528No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT880.11515460814718853No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCG870.11384603305460683No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAC830.10861173268428008No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGTGCAAGCCTGAA830.10861173268428008No Hit
CCTANGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA770.10076028212878997No Hit
CCTANGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA770.10076028212878997No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)