FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54929
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG14202.5851553824027382No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG14112.568770594767791No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC12652.3029729286897633No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC12022.188279415245135No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG11092.0189699430173498No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC10401.8933532378160898No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG10391.8915327058566511No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG9791.7823007882903383No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC9581.7440696171421288No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC8381.5256057820095033No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT7051.2834750314041763No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG6391.1633199220812322No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC6161.1214476870141454No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT6131.11598609113583No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT6021.0959602395820058No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT5961.0850370478253746No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT4280.7791876786396985No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG4250.7737260827613829No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG4250.7737260827613829No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG3990.7263922518159807No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG3750.6826994847894555No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG3740.6808789528300169No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3380.6153398022902292No Hit
CCTANGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC3350.6098782064119136No Hit
CCTACGGGTGGCTGCAGGGAATATTGCTCAATGGGGGAAACCCTGAAGCA3290.5989550146552822No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3260.5934934187769666No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC3260.5934934187769666No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3210.5843907589797739No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3190.5807496950608968No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC3090.5625443754665114No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG3080.5607238435070727No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG3050.5552622476287571No Hit
CCTANGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2850.5188516084399862No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2850.5188516084399862No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2840.5170310764805476No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2820.5133900125616705No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2750.5006462888456007No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2660.48426150121065376No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2590.47151777749458396No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2550.46423564965682973No Hit
CCTACGGGCGGCTGCAGGGAATATTGCTCAATGGGGGAAACCCTGAAGCA2520.4587740537785141No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2480.4514919259407599No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2470.4496713939813214No Hit
CCTANGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2440.44420979810300565No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2410.43874820222469No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2400.43692767026525153No Hit
CCTACGGGGGGCTGCAGGGAATATTGCTCAATGGGGGAAACCCTGAAGCA2330.4241839465491817No Hit
CCTACGGGGGGCAGCAGGGAATATTGCTCAATGGGGGAAACCCTGAAGCA2320.4223634145897431No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2240.40779915891423474No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2240.40779915891423474No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT2200.4005170310764805No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2150.3914143712792878No Hit
CCTANGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2120.38595277540097217No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2080.378670647563218No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2070.37685011560377946No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2060.37502958364434086No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT2050.3732090516849023No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1970.358644796009394No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1970.358644796009394No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1960.35682426404995543No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1960.35682426404995543No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1920.3495421362122012No Hit
CCTANGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1900.3459010722933241No Hit
CCTACGGGAGGCAGCAGGGAATATTGCTCAATGGGGGAAACCCTGAAGCA1890.3440805403338856No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC1850.33679841249613135No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1820.3313368166178157No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1790.32587522073950004No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1780.32405468878006155No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1770.322234156820623No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC1740.3167725609423073No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT1730.3149520289828688No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT1710.3113109650639917No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1670.30402883722623747No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC1670.30402883722623747No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1610.2931056454696062No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1610.2931056454696062No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1550.2821824537129749No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1400.2548744743213967No Hit
CCTANGGGAGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1400.2548744743213967No Hit
CCTANGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1380.2512334104025196No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1360.2475923464836425No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1350.24577181452420396No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC1290.23484862276757268No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT1280.23302809080813416No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1270.2312075588486956No Hit
CCTACGGGAGGCTGCAGGGAATATTGCTCAATGGGGGAAACCCTGAAGCA1250.22756649492981848No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1230.2239254310109414No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC1210.2202843670920643No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1200.21846383513262577No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1170.2130022392543101No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1120.20389957945711737No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1110.2020790474976788No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1080.1966174516193632No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT1060.19297638770048609No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1030.18751479182217043No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1030.18751479182217043No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1000.1820531959438548No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT970.17659160006553914No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC960.1747710681061006No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT960.1747710681061006No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG910.16566840830890786No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC900.16384787634946932No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG890.16202734439003078No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG870.15838628047115366No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC860.15656574851171512No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG850.15474521655227658No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC830.15110415263339946No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG820.14928362067396095No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG820.14928362067396095No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG820.14928362067396095No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT820.14928362067396095No Hit
CCTANGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC820.14928362067396095No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG810.14746308871452238No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC810.14746308871452238No Hit
CCTANGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC800.14564255675508384No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC800.14564255675508384No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG800.14564255675508384No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT770.14018096087676818No Hit
CCTACGGGTGGCAGCAGGGAATATTGCTCAATGGGGGAAACCCTGAAGCA750.1365398969578911No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT740.13471936499845255No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT730.132898833039014No Hit
CCTANGGGTGGCTGCAGGGAATATTGCTCAATGGGGGAAACCCTGAAGCA710.1292577691201369No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC700.12743723716069835No Hit
CCTACGGGGGGCTGNAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC690.1256167052012598No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG670.12197564128238272No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT670.12197564128238272No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT640.11651404540406708No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT610.11105244952575141No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT590.10741138560687433No Hit
CCTACGGGGGGCAGNAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC590.10741138560687433No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT580.10559085364743577No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT580.10559085364743577No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT580.10559085364743577No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT580.10559085364743577No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT570.10377032168799724No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT570.10377032168799724No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC570.10377032168799724No Hit
CCTANGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC560.10194978972855868No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT550.10012925776912013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)