FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54929
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG15942.9019279433450453No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG14012.5505652751734056No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10871.9789182399097014No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10271.8696863223433886No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9961.813249831600794No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG9551.7386080212638135No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG9451.7204027016694279No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8871.614811848021992No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG8501.5474521655227658No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7271.3235267345118242No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7231.31624460667407No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA7011.2761929035664221No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6261.1396530066085309No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5741.0449853447177266No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5731.043164812758288No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5711.039523748839411No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5671.0322416210016567No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5551.010395237488394No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5450.9921899178940087No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG5010.9120865116787126No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA4630.8429062972200477No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA4520.8228804456662236No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4520.8228804456662236No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA4480.8155983178284695No Hit
GACTACAAGGGTATCTAATCCTGTTTACTCCCCACGCTTTCGCACCTGAG3790.6899816126272097No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA3710.6754173569517014No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3660.6663146971545085No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3630.6608531012761929No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG3560.6481093775601231No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3560.6481093775601231No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3490.6353656538440533No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3480.6335451218846146No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3460.6299040579657376No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG3340.6080576744524749No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA3040.5534417156693185No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG3010.5479801197910029No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2940.535236396074933No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2890.5261337362777403No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2740.49882575688616215No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2690.4897230970889694No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG2600.47333830945402244No Hit
GACTACAGGGGTATCTAATCCTGTTTACTCCCCACGCTTTCGCACCTGAG2550.46423564965682973No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2540.4624151176973912No Hit
GACTACTAGGGTATCTAATCCTGTTTACTCCCCACGCTTTCGCACCTGAG2450.44603033006244425No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG2380.4332866063463744No Hit
GACTACACGGGTATCTAATCCTGTTTACTCCCCACGCTTTCGCACCTGAG2370.4314660743869359No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG2360.4296455424274973No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2340.4260044785086202No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2320.4223634145897431No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2300.41872235067086605No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2280.41508128675198896No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2250.40961969087367334No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA2250.40961969087367334No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG2130.3877733073604107No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA2070.37685011560377946No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1970.358644796009394No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1920.3495421362122012No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1850.33679841249613135No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG1810.3295162846583772No Hit
GACTACTCGGGTATCTAATCCTGTTTACTCCCCACGCTTTCGCACCTGAG1770.322234156820623No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1760.32041362486118447No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG1730.3149520289828688No Hit
GACTACCCGGGTATCTAATCCTGTTTACTCCCCACGCTTTCGCACCTGAG1720.31313149702343024No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA1580.2876440495912906No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1550.2821824537129749No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1450.2639771341185895No Hit
GACTACTGGGGTATCTAATCCTGTTTACTCCCCACGCTTTCGCACCTGAG1440.26215660215915093No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG1430.26033607019971233No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG1420.2585155382402738No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1420.2585155382402738No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG1410.25669500628083525No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1370.24941287844308108No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG1350.24577181452420396No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG1340.24395128256476545No Hit
GACTACCAGGGTATCTAATCCTGTTTACTCCCCACGCTTTCGCACCTGAG1290.23484862276757268No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1240.22574596297037994No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1230.2239254310109414No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1200.21846383513262577No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG1130.2057201114165559No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1090.19843798357880174No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG1040.189335323781609No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1000.1820531959438548No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG870.15838628047115366No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA810.14746308871452238No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG780.14200149283620675No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG760.13836042891732964No Hit
GACTACCGGGGTATCTAATCCTGTTTACTCCCCACGCTTTCGCACCTGAG750.1365398969578911No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG740.13471936499845255No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG720.13107830107957547No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG700.12743723716069835No Hit
GANTANAAGGGTATCTAATNCTGTTTGCTCCCCACGCTTTCGAGCCTCAG690.1256167052012598No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG680.12379617324182125No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA680.12379617324182125No Hit
GANTANAAGGGTATCTAATNCTGTTTGCTCCCCACGCTTTCGCGCCTCAG680.12379617324182125No Hit
GANTACAAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGAGCCTCAG660.12015510932294417No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGAGCCTCAG630.11469351344462853No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG620.11287298148518997No Hit
GACTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG590.10741138560687433No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA590.10741138560687433No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG570.10377032168799724No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCGCCTCAG570.10377032168799724No Hit
GANTACAAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCGCCTCAG570.10377032168799724No Hit
GACTANAAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCGCCTCAG550.10012925776912013No Hit
GACTANAAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGAGCCTCAG550.10012925776912013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)