FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004833414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42418
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC12793.0152293837521804No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC11632.741760573341506No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC11502.711113206657551No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC9352.204252911499835No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC8211.9354990805789996No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA6251.473431090574756No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC5681.3390541751143383No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC5651.3319817058795793No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA5371.2659719930218303No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC4961.1693149134801264No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC4841.141025036541091No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT4831.1386675467961715No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA4801.1315950775614128No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC4631.0915177518977792No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT4180.9854307133763969No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA4120.9712857749068792No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT3800.8958461030694518No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT3690.8699137158753358No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC3660.8628412466405772No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC3540.8345513697015419No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA3430.8086189825074261No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3410.8039040030175869No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT3140.7402517799047573No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC3140.7402517799047573No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3060.7213918619454005No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA3020.711961902965722No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC3000.7072469234758829No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC2870.676599556791928No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2680.6318072516384554No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2630.6200198029138573No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2570.6058748644443396No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2490.5870149464849828No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2300.5422226413315102No Hit
CCTANGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2230.525720213117073No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2220.5233627233721534No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT2160.5092177849026357No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2150.5068602951577161No Hit
CCTANGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1990.4691404592390023No Hit
CCTANGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1760.4149181951058513No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGACGCAAGTCTGAT1670.39370078740157477No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1660.3913432976566552No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1630.38427082842189636No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1620.38191333867697674No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1610.3795558489320572No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1600.3771983591871375No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1440.33947852326842376No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGACGCAAGTCTGAT1360.32061860530906694No Hit
CCTANGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1360.32061860530906694No Hit
CCTANGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1350.31826111556414727No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGACGCAAGTCTGAT1340.3159036258192277No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1330.3135461360743081No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1330.3135461360743081No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1320.3111886463293885No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1280.30175868734971No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1260.29704370785987083No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1240.2923287283700316No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1230.289971238625112No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1230.289971238625112No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1210.28525625913527275No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1200.2828987693903532No Hit
CCTANGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1150.2711113206657551No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGACGCAAGTCTGAT1120.26403885143099626No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1110.2616813616860767No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1110.2616813616860767No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1080.25460889245131785No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT1070.25225140270639823No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGACGCAAGTCTGAT1040.2451789334716394No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1030.2428214437267198No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC990.23339148474704136No Hit
CCTANGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA980.23103399500212174No Hit
CCTANGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA960.2263190155122825No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC960.2263190155122825No Hit
CCTANGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC950.22396152576736295No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT910.21453156678768445No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT900.21217407704276486No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT900.21217407704276486No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT890.20981658729784525No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC890.20981658729784525No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC890.20981658729784525No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT880.20745909755292566No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT880.20745909755292566No Hit
CCTANGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC870.20510160780800604No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGACGCAAGTCTGAT860.2027441180630864No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT840.1980291385732472No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC830.1956716488283276No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT810.19095666933848837No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC790.18624168984864917No Hit
CCTANGGGTGGCTGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT790.18624168984864917No Hit
CCTANGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA780.18388420010372955No Hit
CCTANGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC750.17681173086897073No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC740.1744542411240511No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT740.1744542411240511No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT730.1720967513791315No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT730.1720967513791315No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC720.16973926163421188No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT720.16973926163421188No Hit
CCTANGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA720.16973926163421188No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT720.16973926163421188No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT720.16973926163421188No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT720.16973926163421188No Hit
CCTANGGGGGGCAGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT710.1673817718892923No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC710.1673817718892923No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT700.16502428214437267No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC700.16502428214437267No Hit
CCTANGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC690.16266679239945306No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT690.16266679239945306No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT690.16266679239945306No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC690.16266679239945306No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT690.16266679239945306No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA690.16266679239945306No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT680.16030930265453347No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT680.16030930265453347No Hit
CCTANGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC670.15795181290961385No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT660.15559432316469424No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT650.15323683341977462No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGTGCAAGCCTGAA650.15323683341977462No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT650.15323683341977462No Hit
CCTANGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC640.150879343674855No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT640.150879343674855No Hit
CCTANGGGGGGCTGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT640.150879343674855No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGTGCAAGCCTGAA630.14852185392993542No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGTGCAAGCCTGAA630.14852185392993542No Hit
CCTANGGGCGGCTGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT630.14852185392993542No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT600.1414493846951766No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT600.1414493846951766No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT590.13909189495025698No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT590.13909189495025698No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT580.13673440520533736No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT570.13437691546041775No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT570.13437691546041775No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT560.13201942571549813No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGTGCAAGCCTGAA550.12966193597057854No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT530.12494695648073931No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT510.12023197699090007No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC500.11787448724598047No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT480.11315950775614125No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC460.10844452826630205No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT450.10608703852138243No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT450.10608703852138243No Hit
CCTANGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA440.10372954877646283No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGACGCAAGTCTGAT440.10372954877646283No Hit
CCTANGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC430.1013720590315432No Hit
CCTANGGGAGGCAGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT430.1013720590315432No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)