FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142477
Sequences flagged as poor quality0
Sequence length301
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC51083.585140057693523No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC47833.3570330649859272No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC43193.0313664661664688No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC40482.8411603276318282No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT37912.660780336475361No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC34112.3940706219249424No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT32012.2466784112523426No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT29902.0985843329098732No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT27591.9364529011700136No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC24691.7329112769078516No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT23141.6241217880780756No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT18831.3216168223643114No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT16801.1791376853807984No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT16401.1510629785860174No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT16221.138429360528366No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT14230.9987576942243309No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC13360.9376952069456825No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT12180.8548748219010787No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC10770.755911480449476No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC9910.6955508608406971No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT9060.6358921089017876No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8960.6288734322030924No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8930.6267678291934838No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT8900.6246622261838753No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7740.5432455764790106No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7640.5362268997803155No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7540.5292082230816202No Hit
CCTACGGGTGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA7450.5228914140527945No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT7440.522189546382925No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7150.5018353839567088No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6940.48709616288944885No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6450.4527046470658422No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6180.4337542199793651No Hit
CCTACGGGGGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA5970.41901499891210514No Hit
CCTACGGGCGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA5960.41831313124223557No Hit
CCTACGGGGGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA5930.416207528232627No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5850.41059258687367084No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5610.3937477627968023No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5550.38953655677758514No Hit
CCTACGGGAGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA5210.3656730560020214No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4750.3333871431880233No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4720.33128154017841477No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4610.32356099580985004No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4120.28916947998624337No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3850.2702190528997663No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3830.2688153175600272No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3510.2463555521242025No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3260.22880886037746442No Hit
CCTACGGGAGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA3180.22319391901850827No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3080.216175242319813No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2830.19862855057307494No Hit
CCTACGGGTGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT2720.1909080062045102No Hit
CCTACGGGTGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT2580.18108185882633687No Hit
CCTACGGGGGGCAGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT2580.18108185882633687No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG2490.17476504979751117No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2370.1663426377590769No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2370.1663426377590769No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG2330.1635351670795988No Hit
CCTACGGGGGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT2290.16072769640012072No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2280.16002582873025117No Hit
CCTACGGGGGGCAGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT2230.1565164903809036No Hit
CCTANGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2200.15441088737129502No Hit
CCTACGGGTGGCTGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC2170.15230528436168644No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2050.14388287232325217No Hit
CCTACGGGTGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA2000.14037353397390456No Hit
CCTACGGGCGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT1980.1389697986341655No Hit
CCTACGGGGGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1950.13686419562455693No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1930.1354604602848179No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1930.1354604602848179No Hit
CCTACGGGGGGCAGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC1920.13475859261494838No Hit
CCTACGGGCGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1910.13405672494507886No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1910.13405672494507886No Hit
CCTACGGGCGGCTGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC1860.13054738659573126No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1860.13054738659573126No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1850.1298455189258617No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1780.12493244523677507No Hit
CCTACGGGAGGCAGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT1780.12493244523677507No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1760.12352870989703602No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1750.1228268422271665No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1720.12072123921755791No Hit
CCTACGGGGGGCTGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC1700.11931750387781886No Hit
CCTACGGGTGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1670.11721190086821032No Hit
CCTACGGGTGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1650.11580816552847126No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1600.11229882717912365No Hit
CCTACGGGAGGCAGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1590.11159695950925413No Hit
CCTANGGGTGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA1580.1108950918393846No Hit
CCTANGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1550.10878948882977603No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1510.10598201815029795No Hit
CCTANGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1500.10528015048042842No Hit
CCTACGGGCGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA1470.10317454747081985No Hit
CCTACGGGGGGCAGCAGGCAGCAGGCAGCGCGCGGTACTGGCGAGTTCTC1460.10247267980095032No Hit
CCTANGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1450.10177081213108082No Hit
CCTACGGGGGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1430.10036707679134177No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)