FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142477
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA55923.9248440099103714No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA38672.7141222793854447No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA37872.657972865795883No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG37162.608140261235147No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA33902.3793314008576822No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA29512.0712114937849617No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG25721.8052036469044126No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA24821.7420355566161556No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG24611.7272963355488957No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG21691.522350975946995No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG20151.4142633547870884No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA20131.4128596194473495No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG19661.3798718389634819No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA19451.3651326178962218No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG16991.1924731711083194No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG14511.0184099889806775No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG13750.9650680460705938No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG13560.9517325603430729No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG12920.9068130294714235No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG11880.8338187918049932No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA11810.8289057181159064No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG10900.7650357601577799No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10550.7404703917123465No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8860.6218547555043972No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA7550.5299100907514896No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG7440.522189546382925No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7260.5095559283252736No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7150.5018353839567088No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG7110.49902791327723073No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7030.49341297191827455No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG6120.429543013960148No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG5260.36918239435136896No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5220.36637492367189095No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG4830.3390020845469795No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4740.3326852755181538No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4690.32917593716880617No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4370.30671617173298144No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4210.29548628901506907No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4090.2870638769766348No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG3870.2716227882395053No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG3810.2674115822202882No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3380.23723127241589873No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3350.23512566940629012No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3270.22951072804733397No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3100.21757897765955206No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2610.18318746183594548No Hit
GACTACAAGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC2580.18108185882633687No Hit
GACTACAAGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT2550.1789762558167283No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2550.1789762558167283No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG2240.1572183580507731No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2190.1537090197014255No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2050.14388287232325217No Hit
GANTACAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2020.14177726931364362No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1990.13967166630403505No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1930.1354604602848179No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1910.13405672494507886No Hit
GACTACTAGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1860.13054738659573126No Hit
GACTACACGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1860.13054738659573126No Hit
GACTACACGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1850.1298455189258617No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1830.12844178358612268No Hit
GACTACAAGGGTATCTAATCCATTATTTGAAGCGGGCTCGGAGGAAACGT1830.12844178358612268No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1820.12773991591625314No Hit
GACTACAGGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1770.12423057756690555No Hit
GACTACAGGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1750.1228268422271665No Hit
GANTANAAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1740.12212497455729697No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1710.1200193715476884No Hit
GANTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1670.11721190086821032No Hit
GACTACTCGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1580.1108950918393846No Hit
GACTACAAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1510.10598201815029795No Hit
GACTACTAGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1480.10387641514068938No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1460.10247267980095032No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1450.10177081213108082No Hit
GACTACTCGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1440.10106894446121129No Hit
GACTACAGGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1440.10106894446121129No Hit
GACTACAAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1430.10036707679134177No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)