FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004833422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10546
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC4143.925659017637019No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2852.7024464251849043No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2492.3610847714773375No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2382.2567798217333586No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2071.9628295088185095No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1531.450787028257159No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1030.976673620329983No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC990.9387445476958088No Hit
CCTACGGGGGGCAGCAGTAAATGAACGGTTGAGCGGTAAAGCGGATTAGA860.815475061634743No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC820.7775459890005689No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT800.7585814526834819No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT750.7111701118907643No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT740.7016878437322208No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT730.6922055755736772No Hit
CCTACGGGAGGCAGCAGTAAATGAACGGTTGAGCGGTAAAGCGGATTAGA690.6542765029395031No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT690.6542765029395031No Hit
CCTACGGGGGGCTGCAGTAAATGAACGGTTGAGCGGTAAAGCGGATTAGA650.616347430305329No Hit
CCTACGGGTGGCTGCAGTAAATGAACGGTTGAGCGGTAAAGCGGATTAGA650.616347430305329No Hit
CCTACGGGCGGCTGCAGTAAATGAACGGTTGAGCGGTAAAGCGGATTAGA640.6068651621467855No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT630.597382893988242No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT610.5784183576711549No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT610.5784183576711549No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT610.5784183576711549No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT570.5404892850369808No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT560.5310070168784373No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT550.5215247487198937No Hit
CCTANGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC540.5120424805613503No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT530.5025602124028068No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT500.4741134079271762No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT490.4646311397686327No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT480.45514887161008916No Hit
CCTANGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC470.44566660345154563No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT450.4267020671344586No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT450.4267020671344586No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT440.41721979897591505No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT430.4077375308173715No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT410.38877299450028446No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT410.38877299450028446No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT410.38877299450028446No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT400.37929072634174094No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT380.36032619002465394No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT380.36032619002465394No Hit
CCTANGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC380.36032619002465394No Hit
CCTANGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC370.3508439218661104No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC350.33187938554902335No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT350.33187938554902335No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT340.3223971173904798No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT340.3223971173904798No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT330.3129148492319363No Hit
CCTACGGGAGGCTGCAGTAAATGAACGGTTGAGCGGTAAAGCGGATTAGA330.3129148492319363No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT320.30343258107339277No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT310.29395031291484924No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT310.29395031291484924No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT300.2844680447563057No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC290.2749857765977622No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT280.26550350843921866No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT280.26550350843921866No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT280.26550350843921866No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT280.26550350843921866No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT270.25602124028067513No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT270.25602124028067513No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC270.25602124028067513No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT270.25602124028067513No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC260.24653897212213163No Hit
CCTANGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC260.24653897212213163No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT250.2370567039635881No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT240.22757443580504458No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC240.22757443580504458No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT240.22757443580504458No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT240.22757443580504458No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCT230.21809216764650105No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT230.21809216764650105No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCG220.20860989948795752No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCG190.18016309501232697No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCT190.18016309501232697No Hit
CCTACGGGCGGCAGCAGTAAATGAACGGTTGAGCGGTAAAGCGGATTAGA190.18016309501232697No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT190.18016309501232697No Hit
CCTANGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC190.18016309501232697No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCT180.17068082685378344No Hit
CCTANGGGGGGCAGCAGTAAATGAACGGTTGAGCGGTAAAGCGGATTAGA180.17068082685378344No Hit
CCTACGGGTGGCAGCAGTAAATGAACGGTTGAGCGGTAAAGCGGATTAGA180.17068082685378344No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT170.1611985586952399No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT170.1611985586952399No Hit
CCTANGGGGGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT170.1611985586952399No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCT170.1611985586952399No Hit
CCTACGGGGGGCAGNAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC170.1611985586952399No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT170.1611985586952399No Hit
CCTANGGGGGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT160.15171629053669639No Hit
CCTANGGGGGGCTGCAGTAAATGAACGGTTGAGCGGTAAAGCGGATTAGA150.14223402237815286No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT150.14223402237815286No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT150.14223402237815286No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCC150.14223402237815286No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCG140.13275175421960933No Hit
CCTANGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC140.13275175421960933No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCC140.13275175421960933No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC140.13275175421960933No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT140.13275175421960933No Hit
CCTANGGGGGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT140.13275175421960933No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT130.12326948606106582No Hit
CCTANGGGGGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT130.12326948606106582No Hit
CCTANGGGCGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT130.12326948606106582No Hit
CCTANGGGCGGCTGCAGTAAATGAACGGTTGAGCGGTAAAGCGGATTAGA130.12326948606106582No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT130.12326948606106582No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC130.12326948606106582No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCC130.12326948606106582No Hit
CCTACGGGGGGCAGCAGAGCTGAATAAGGATCTGAACGAGAGAAGGCTGT120.11378721790252229No Hit
CCTANGGGGGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT120.11378721790252229No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT120.11378721790252229No Hit
CCTACGGGGGGCTGCAGGCAAACCGTGAATCTCCTAGTTATCTACATCTG120.11378721790252229No Hit
CCTANGGGTGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT120.11378721790252229No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT120.11378721790252229No Hit
CCTANGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC120.11378721790252229No Hit
CCTACGGGTGGCTGCAGGCAAACCGTGAATCTCCTAGTTATCTACATCTG120.11378721790252229No Hit
CCTANGGGTGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT120.11378721790252229No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT120.11378721790252229No Hit
CCTANGGGGGGCTGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT120.11378721790252229No Hit
CCTANGGGAGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT110.10430494974397876No Hit
CCTANGGGGGGCAGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT110.10430494974397876No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCG110.10430494974397876No Hit
CCTANGGGAGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT110.10430494974397876No Hit
CCTANGGGGGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT110.10430494974397876No Hit
CCTACGGGGGGCAGCAGCTCGGAGATGTGGATTAGATACCCTTGTAGTCC110.10430494974397876No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT110.10430494974397876No Hit
CCTACGGGGGGCTGCAGAGCTGAATAAGGATCTGAACGAGAGAAGGCTGT110.10430494974397876No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)