FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004833429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11940
Sequences flagged as poor quality0
Sequence length37-251
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2772.319932998324958No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2682.2445561139028474No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1861.5577889447236182No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1841.541038525963149No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1841.541038525963149No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1791.4991624790619766No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1731.4489112227805694No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1641.373534338358459No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1631.3651591289782243No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1581.3232830820770518No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1561.306532663316583No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1501.256281407035176No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1481.2395309882747068No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1361.139028475711893No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1301.0887772194304857No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1291.0804020100502512No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1291.0804020100502512No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1271.0636515912897822No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1251.0469011725293134No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1180.9882747068676716No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1110.9296482412060301No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1030.8626465661641541No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCTCTCAG950.7956448911222781No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG940.7872696817420435No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG940.7872696817420435No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG910.7621440536013401No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG910.7621440536013401No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG900.7537688442211055No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG860.7202680067001676No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG840.7035175879396985No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCTCTCAG820.6867671691792295No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG800.6700167504187605No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG800.6700167504187605No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG790.6616415410385259No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG780.6532663316582915No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG750.628140703517588No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG690.5778894472361809No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG670.5611390284757118No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG640.5360134003350084No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG640.5360134003350084No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG630.5276381909547738No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG620.5192629815745394No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG620.5192629815745394No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG610.5108877721943048No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCTCTCAG600.5025125628140703No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG550.46063651591289784No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG540.4522613065326633No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCTCTCAG540.4522613065326633No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG520.43551088777219427No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG520.43551088777219427No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG510.4271356783919598No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG510.4271356783919598No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG500.41876046901172526No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG500.41876046901172526No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG500.41876046901172526No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG490.4103852596314908No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCTCTCAG480.4020100502512563No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG470.39363484087102174No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCTCTCAG470.39363484087102174No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG470.39363484087102174No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG460.38525963149078724No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCTCTCAG450.37688442211055273No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG450.37688442211055273No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG440.3685092127303182No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG410.34338358458961477No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG370.3098827470686767No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG360.3015075376884422No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG360.3015075376884422No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG360.3015075376884422No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCTCTCAG350.2931323283082077No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG340.28475711892797323No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG330.2763819095477387No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG330.2763819095477387No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG330.2763819095477387No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG330.2763819095477387No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG320.2680067001675042No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG320.2680067001675042No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG310.2596314907872697No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG310.2596314907872697No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG300.25125628140703515No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG300.25125628140703515No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG290.24288107202680068No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG290.24288107202680068No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG280.23450586264656617No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACATGAG280.23450586264656617No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG280.23450586264656617No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG280.23450586264656617No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG280.23450586264656617No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG270.22613065326633164No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG270.22613065326633164No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACATGAG260.21775544388609713No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG260.21775544388609713No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG260.21775544388609713No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG260.21775544388609713No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG250.20938023450586263No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACATGAG250.20938023450586263No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG250.20938023450586263No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG250.20938023450586263No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG240.20100502512562815No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG240.20100502512562815No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG240.20100502512562815No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG240.20100502512562815No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACATGAG230.19262981574539362No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG230.19262981574539362No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG220.1842546063651591No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG210.17587939698492464No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG210.17587939698492464No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG200.16750418760469013No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG200.16750418760469013No Hit
GACTACAAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCACCCGTAGG200.16750418760469013No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCTCTCAG200.16750418760469013No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG200.16750418760469013No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATGAG200.16750418760469013No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG190.1591289782244556No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG190.1591289782244556No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG190.1591289782244556No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG190.1591289782244556No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCCCCCGTAGG180.1507537688442211No Hit
GACTACAAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCGCCCGTAGG170.14237855946398661No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG170.14237855946398661No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG170.14237855946398661No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG170.14237855946398661No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG160.1340033500837521No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG160.1340033500837521No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG160.1340033500837521No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG150.12562814070351758No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACATGAG150.12562814070351758No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG150.12562814070351758No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCTGGCTTTCGCGCCTCAG150.12562814070351758No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG150.12562814070351758No Hit
GACTACACGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG150.12562814070351758No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG150.12562814070351758No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG150.12562814070351758No Hit
GACTACAAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCTCCCGTAGG140.11725293132328309No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG140.11725293132328309No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA140.11725293132328309No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG140.11725293132328309No Hit
GACTACAAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCCCCCGTAGG130.10887772194304857No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG130.10887772194304857No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG130.10887772194304857No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG130.10887772194304857No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG120.10050251256281408No Hit
GACTACAAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCCCCCGTAGG120.10050251256281408No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG120.10050251256281408No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG120.10050251256281408No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCTGGCTTTCGCGCCTCAG120.10050251256281408No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA120.10050251256281408No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG120.10050251256281408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)