FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64933
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG12801.9712626861534195No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG9381.444565937196803No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG9121.4045246638843114No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG8841.3614032926247055No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7991.23049912987233No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7321.127315848643987No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG7041.0841944773843808No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6631.021052469468529No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG6210.9563704125791199No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6150.9471301187377759No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG5170.7962053193291546No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5130.7900451234349253No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5020.7731045847257942No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5020.7731045847257942No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4980.7669443888315648No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG4900.754623997043106No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4900.754623997043106No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4890.7530839480695486No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCCTTCGCTCCTCAG4860.7484638011488766No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4840.7453837032017618No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG4720.7269031155190735No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4430.68224169528591No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC4380.6745414504181233No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4240.6529807647883202No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4230.6514407158147629No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4060.6252598832642878No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGGGCCTCAA4020.6190996873700584No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3900.60061909968737No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3860.5944589037931406No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG3720.5728982181633375No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3690.5682780712426655No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3570.5497974835599772No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG3450.5313168958772889No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCCTTCGCTCCTCAG3380.5205365530623873No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCCTTCGCTCCTCAG3250.5005159164061418No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3200.49281567153835487No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG3200.49281567153835487No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3200.49281567153835487No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG3170.48819552461768284No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3170.48819552461768284No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGGGCCTCAA3140.4835753776970108No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCCTTCGCTCCTCAG2910.4481542513051915No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2870.4419940554109621No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2790.42967366362250314No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCCTTCGCTCCTCAG2780.4281336146489459No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2770.4265935656753885No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2750.42351346772827375No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG2740.4219734187547164No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2730.4204333697811591No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2700.41581322286048694No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2690.4142731738869296No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGGGCCTCAA2650.4081129779927002No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2640.40657292901914277No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2590.398872684151356No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2580.3973326351777986No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2420.3726918516008809No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2400.3696117536537662No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2390.36807170468020883No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2330.35883141083886466No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGGGCCTCAA2310.35575131289174994No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2310.35575131289174994No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2300.35421126391819263No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2290.35267121494463527No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2210.3403508231561764No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2100.3234102844470454No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGGGCCTCAA2090.3218702354734881No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2070.3187901375263733No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2050.31571003957925864No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2050.31571003957925864No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2010.3095498436850292No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCCTTCGCTCCTCAG1990.30646974573791447No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG1870.2879891580552262No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1870.2879891580552262No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1830.28182896216099673No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1830.28182896216099673No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1830.28182896216099673No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGGGCCTCAA1800.27720881524032465No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1790.2756687662667673No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1780.2741287172932099No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1780.2741287172932099No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCCTTCGCTCCTCAG1740.2679685213989805No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1730.2664284724254231No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1710.2633483744783084No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1710.2633483744783084No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1620.2494879337162922No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1590.2448677867956201No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGGGCCTCAA1570.2417876888485054No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGGGCCTCAA1560.24024763987494802No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG1540.23716754192783332No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCCTTCGCTCCTCAG1510.23254739500716123No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1500.23100734603360384No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1490.2294672970600465No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT1480.22792724808648915No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1470.22638719911293176No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1440.22176705219225973No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT1410.21714690527158764No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1380.21252675835091556No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1370.2109867093773582No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1360.20944666040380086No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG1330.20482651348312877No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1310.20174641553601405No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1300.2002063665624567No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCCAGCTTTCGCGCCTCAG1270.1955862196417846No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1250.1925061216946699No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1230.18942602374755516No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT1220.18788597477399782No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1220.18788597477399782No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1220.18788597477399782No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1210.18634592580044046No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1170.180185729906211No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG1160.17864568093265365No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1160.17864568093265365No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1150.17710563195909632No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1140.17556558298553893No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT1130.1740255340119816No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1120.17248548503842423No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1070.16478524017063742No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCCAGCTTTCGCGCCTCAG1030.158625044276408No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG990.15246484838217855No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG970.14938475043506383No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT970.14938475043506383No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCCAGCTTTCGCGCCTCAG960.14784470146150647No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA940.14476460351439177No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA940.14476460351439177No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG940.14476460351439177No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCCAGCTTTCGCGCCTCAG930.14322455454083438No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG920.14168450556727705No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG920.14168450556727705No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCCTTCGCTCCTCAG890.13706435864660496No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG870.13398426069949024No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCTCCCGTAGGCTGTCTCT860.13244421172593288No Hit
GACTACAGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT840.12936411377881815No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG830.12782406480526082No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG830.12782406480526082No Hit
GACTACACGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT820.12628401583170346No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT820.12628401583170346No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG820.12628401583170346No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGGGCCTCAA810.1247439668581461No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCCAGCTTTCGCGCCTCAG800.12320391788458872No Hit
GACTACAGGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT790.12166386891103137No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT770.11858377096391666No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG770.11858377096391666No Hit
GACTACACGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT770.11858377096391666No Hit
GACTACACGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT760.1170437219903593No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA760.1170437219903593No Hit
GACTACACGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT750.11550367301680192No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG750.11550367301680192No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT750.11550367301680192No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT730.11242357506968723No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA720.11088352609612986No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT720.11088352609612986No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG720.11088352609612986No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA680.10472333020190043No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT680.10472333020190043No Hit
GACTACACGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT680.10472333020190043No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG670.10318328122834305No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG650.10010318328122834No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA650.10010318328122834No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)