FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119055
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG53544.49708118096678No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG36203.0406114820881105No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG36183.0389315862416533No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG33062.77686783419428No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG28632.4047709042039394No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG26952.2636596531015076No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG22951.927680483810004No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG19491.637058502372853No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG18671.5681827726680946No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA18451.549703918357062No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG17851.4993070429633362No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG17821.49678719919365No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG17751.4909075637310487No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG16031.346436520935702No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG14261.1977657385242115No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA12731.0692537062702112No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA12461.046575112343035No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG12371.0390155810339758No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG12351.0373356851875184No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG11820.9928184452563941No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG11650.9785393305615052No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG11010.9247826634748645No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA10570.8878249548527992No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA10080.8466675066145899No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9680.8130695896854395No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9670.8122296417622108No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG9660.811389693838982No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA8490.713115786821217No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG7550.6341606820377137No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA6720.5644450044097267No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG6570.5518457855612952No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA6420.5392465667128638No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG5900.49556927470496825No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5570.4678509932384192No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG4140.3477384402167066No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3650.30658099197849736No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2780.23350552265759525No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2100.17638906387803957No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2080.17470916803158204No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1930.16210994918315064No Hit
GACTANAAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG1890.1587501574902356No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1870.15707026164377808No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1790.150350678257948No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1760.14783083448826173No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1760.14783083448826173No Hit
GANTACAAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG1720.14447104279534667No Hit
GACTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1670.1402713031792029No Hit
GANTANAAGGGTATCTAATNCTGTTTGCTCCCCACGCTTTCGCACCTCAG1660.13943135525597414No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1590.1335517197933728No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1540.12935198017722901No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1500.12599218848431398No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG1470.12347234471462769No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1420.1192726050984839No Hit
GACTANAGGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG1390.11675276132879762No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1340.11255302171265381No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1320.11087312586619628No Hit
GACTANACGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG1320.11087312586619628No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1320.11087312586619628No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1300.10919323001973877No Hit
GANTANAGGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG1300.10919323001973877No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1280.10751333417328127No Hit
GANTANAGGGGTATCTAATNCTGTTTGCTCCCCACGCTTTCGCACCTCAG1270.1066733862500525No Hit
GACTANTAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG1270.1066733862500525No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1270.1066733862500525No Hit
GANTACTAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG1250.10499349040359499No Hit
GACTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1230.10331359455713746No Hit
GANTANACGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG1230.10331359455713746No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1230.10331359455713746No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1230.10331359455713746No Hit
GANTACAGGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG1220.10247364663390869No Hit
GANTANACGGGTATCTAATNCTGTTTGCTCCCCACGCTTTCGCACCTCAG1220.10247364663390869No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1200.10079375078745118No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1200.10079375078745118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)