FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144922
Sequences flagged as poor quality0
Sequence length301
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC99386.857481955810712No Hit
CCTACGGGGGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC98706.810560163398242No Hit
CCTACGGGGGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC83655.772070493092836No Hit
CCTACGGGCGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC76105.251100592042616No Hit
CCTACGGGAGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC66824.6107561308842No Hit
CCTACGGGAGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC46043.1768813568678325No Hit
CCTACGGGTGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC26301.814769324188184No Hit
CCTACGGGCGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC19861.3703923489877314No Hit
CCTANGGGTGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC18231.2579180524696043No Hit
CCTANGGGGGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC17361.1978857592360028No Hit
CCTANGGGGGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC16531.140613571438429No Hit
CCTANGGGCGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC14330.9888077724569079No Hit
CCTANGGGAGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC12100.834931894398366No Hit
CCTACGGGTGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC9640.6651854100826651No Hit
CCTACGGGGGGCAGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC8850.6106733277211189No Hit
CCTANGGGAGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC8780.6058431432080705No Hit
CCTACGGGGGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC8240.5685817198216971No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC7570.5223499537682339No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC7420.5119995583831303No Hit
CCTACGGGCGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC7130.49198879397192974No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC6270.43264652709733514No Hit
CCTACGGGAGGCAGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC6230.4298864216613075No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC5390.37192420750472666No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC4970.3429431004264363No Hit
CCTANGGGTGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC4470.30844178247609055No Hit
CCTACGGGAGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC4370.30154151888602143No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT3950.27256041180773105No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT3860.2663501745766688No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC3810.26290004278163426No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG3800.2622100164226273No Hit
CCTANGGGCGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC3610.24909951560149599No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG3570.24633941016546831No Hit
CCTACGGGTGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC3470.2394391465753992No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT3240.22356854031824014No Hit
CCTACGGGTGGCTGCAGAGTGTAGGAGTACTCTAGGATAAGTGGGCATAT3230.22287851395923325No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3150.21735830308717793No Hit
CCTACGGGGGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC3100.21390817129214335No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG3060.21114806585611573No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT3000.2070079077020742No Hit
CCTACGGGGGGCAGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC2990.20631788134306733No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2940.20286774954803274No Hit
CCTACGGGCGGCTGCAGAGTGTAGGAGTACTCTAGGATAAGTGGGCATAT2930.2021777231890258No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG2920.20148769683001894No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2870.19803756503498435No Hit
CCTACGGGGGGCAGCAGAGTGTAGGAGTACTCTAGGATAAGTGGGCATAT2750.18975724872690136No Hit
CCTACGGGTGGCTGCAGTTGCAACGGAGGAATTCTGTGGTGTTCCCGGTT2680.18492706421385297No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2680.18492706421385297No Hit
CCTACGGGGGGCTGCAGAGTGTAGGAGTACTCTAGGATAAGTGGGCATAT2520.17388664246974234No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2510.17319661611073545No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT2420.16698637887967321No Hit
CCTACGGGTGGCAGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC2420.16698637887967321No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG2400.16560632616165938No Hit
CCTACGGGCGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC2390.16491629980265246No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2370.16353624708463865No Hit
CCTACGGGGGGCTGCAGTTGCAACGGAGGAATTCTGTGGTGTTCCCGGTT2360.1628462207256317No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2350.16215619436662482No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTATCCG2350.16215619436662482No Hit
CCTACGGGGGGCAGCAGTTGCAACGGAGGAATTCTGTGGTGTTCCCGGTT2350.16215619436662482No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2280.15732600985357642No Hit
CCTACGGGCGGCTGCAGTTGCAACGGAGGAATTCTGTGGTGTTCCCGGTT2250.15525593077655567No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2240.15456590441754875No Hit
CCTACGGGGGGCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCG2220.15318585169953491No Hit
CCTACGGGTGGCTGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCG2210.15249582534052802No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTATCCG2160.14904569354549344No Hit
CCTACGGGAGGCAGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC2120.1462855881094658No Hit
CCTACGGGAGGCAGCAGAGTGTAGGAGTACTCTAGGATAAGTGGGCATAT2110.14559556175045885No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2100.14490553539145196No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1980.136625219083369No Hit
CCTACGGGGGGCTGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCG1970.13593519272436205No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT1970.13593519272436205No Hit
CCTACGGGCGGCAGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC1940.13386511364734133No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1930.1331750872883344No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTATCCG1870.12903492913429293No Hit
CCTACGGGTGGCTGCAGAGAGTGGGGGAGTTGGGGTGGTGGGGTGCTGGT1860.128344902775286No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1840.12696485005727218No Hit
CCTACGGGGGGCAGCAGATCTTTCTGTACTATACCTAAGCCAAGCACGAT1720.11868453374918922No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG1690.11661445467216847No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1690.11661445467216847No Hit
CCTACGGGGGGCTGCAGATCTTTCTGTACTATACCTAAGCCAAGCACGAT1680.11592442831316156No Hit
CCTACGGGGGGCAGCAGAGAGTGGGGGAGTTGGGGTGGTGGGGTGCTGGT1680.11592442831316156No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1650.11385434923614082No Hit
CCTACGGGAGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC1640.1131643228771339No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1610.11109424380011317No Hit
CCTANGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1610.11109424380011317No Hit
CCTANGGGGGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC1600.11040421744110626No Hit
CCTACGGGAGGCTGCAGAGTGTAGGAGTACTCTAGGATAAGTGGGCATAT1570.10833413836408551No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTATCCG1550.10695408564607167No Hit
CCTACGGGCGGCTGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCG1540.10626405928706477No Hit
CCTANGGGTGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC1530.10557403292805786No Hit
CCTACGGGCGGCTGCAGATCTTTCTGTACTATACCTAAGCCAAGCACGAT1520.10488400656905093No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1520.10488400656905093No Hit
CCTACGGGAGGCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCG1490.1028139274920302No Hit
CCTACGGGTGGCTGCAGATCTTTCTGTACTATACCTAAGCCAAGCACGAT1480.10212390113302328No Hit
CCTACGGGGGGCAGCAGGGGGACTGGAGGTGCCCAAGTCAGTTAGAGCCA1480.10212390113302328No Hit
CCTACGGGTGGCTGCAGGGGGACTGGAGGTGCCCAAGTCAGTTAGAGCCA1470.10143387477401637No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)