FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144922
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT110217.604780502615199No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT73505.0716937387008185No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT71614.9412787568485115No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT65284.504492071597134No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT57333.9559211161866386No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT48203.3259270504133256No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT40192.7732159368487874No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT38042.6248602696623013No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT23591.6277721808973102No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA10720.7397082568554119No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG10590.730737914188322No Hit
GACTACAAGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG9560.6596651992106098No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7220.49819903120299197No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7080.4885386621768952No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6950.4795683195098053No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6800.4692179241247016No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6340.4374767116103835No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6300.43471660617435587No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6070.41884599991719684No Hit
GACTACACGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG6070.41884599991719684No Hit
GACTACAGGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG6060.4181559735581899No Hit
GACTACTAGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG5550.3829646292488373No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5410.3733042602227405No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4940.34087302134941555No Hit
GACTACTCGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG4670.3222423096562289No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4550.3139619933481459No Hit
GACTACCCGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG4320.29809138709098687No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4040.2787706490387933No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4010.2767005699617725No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4010.2767005699617725No Hit
GANTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT3910.2698003063717034No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3760.2594499109865997No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT3720.256689805550572No Hit
GANTANAAGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT3650.25185962103752363No Hit
GANTACAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT3640.25116959467851674No Hit
GACTANAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT3420.2359890147803646No Hit
GACTACTGGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG3290.22701867211327473No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3250.22425856667724706No Hit
GACTACAAGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA3190.22011840852320558No Hit
GACTACCAGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG3180.21942838216419866No Hit
GACTACAAGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT3120.21528822401015718No Hit
GACTACAAGGGTATCTAATCCCGAGCTTAGACAAAGCCACTGCCTGGGTA3060.21114806585611573No Hit
GANTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2920.20148769683001894No Hit
GANTACAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2870.19803756503498435No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2730.18837719600888753No Hit
GACTACAGGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT2730.18837719600888753No Hit
GANTANAGGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2640.18216695877782532No Hit
GANTACACGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2540.17526669518775617No Hit
GACTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2540.17526669518775617No Hit
GACTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2520.17388664246974234No Hit
GANTANACGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2470.17043651067470778No Hit
GACTACTAGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT2410.16629635252066627No Hit
GACTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2400.16560632616165938No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2390.16491629980265246No Hit
GACTACACGGGTATCTAATCCCGAGCTTAGACAAAGCCACTGCCTGGGTA2390.16491629980265246No Hit
GANTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2370.16353624708463865No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2360.1628462207256317No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2290.1580160362125833No Hit
GACTANAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2260.15594595713556259No Hit
GACTACAGGGGTATCTAATCCCGAGCTTAGACAAAGCCACTGCCTGGGTA2250.15525593077655567No Hit
GANTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2250.15525593077655567No Hit
GANTANTAGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2210.15249582534052802No Hit
GACTANACGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2190.1511157726225142No Hit
GACTANTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2180.15042574626350724No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2130.14697561446847268No Hit
GACTACCGGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG2120.1462855881094658No Hit
GACTACACGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT2080.14352548267343812No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2080.14352548267343812No Hit
GANTANTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2060.14214542995542429No Hit
GANTACTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2050.1414554035964174No Hit
GANTANTCGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2030.14007535087840356No Hit
GACTANTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1990.1373152454423759No Hit
GACTACAGGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA1960.13524516636535516No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1950.13455514000634825No Hit
GACTACAAGGGTATCTAATCCAAAAACTGGTGTTTGGCAAGGCCAGTAAT1900.13110500821131366No Hit
GANTACTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1890.13041498185230677No Hit
GACTACACGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA1870.12903492913429293No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1860.128344902775286No Hit
GACTANTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1840.12696485005727218No Hit
GACTACTAGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA1820.12558479733925837No Hit
GANTANCCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1810.12489477098025145No Hit
GACTACTAGGGTATCTAATCCCGAGCTTAGACAAAGCCACTGCCTGGGTA1790.12351471826223762No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1780.1228246919032307No Hit
GACTANCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1780.1228246919032307No Hit
GACTANCCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1680.11592442831316156No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1680.11592442831316156No Hit
GACTACAAGGGTATCTAATCCATTGCCCCTACCCCTGCCCCCAATTCCCC1660.11454437559514773No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1650.11385434923614082No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1640.1131643228771339No Hit
GACTACTCGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT1600.11040421744110626No Hit
GANTANCCGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1600.11040421744110626No Hit
GANTACCCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1600.11040421744110626No Hit
GANTACTGGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1590.10971419108209933No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1580.10902416472309243No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1560.10764411200507859No Hit
GACTACCCGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA1510.10419398021004403No Hit
GACTACAAGGGTATCTAATCCAGTGTCTGTTACAACACATTCTGAGGTCT1480.10212390113302328No Hit
GACTACAAGGGTATCTAATCCCTAGAACTCCGAGTGCAGCAGATGGTTTC1450.10005382205600254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)