FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141195
Sequences flagged as poor quality0
Sequence length301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC43483.0794291582563122No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC38152.701937037430504No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC35282.49867204929353No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC33482.3711887814724317No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC29122.06239597719466No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC20651.4625163780587132No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA20161.427812599596303No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT17671.2514607457771167No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA16281.1530153334041573No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA15931.1282269202167217No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT15731.114062112681044No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA15041.0651935266829562No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT14661.0382803923651687No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC14401.0198661425687878No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13570.9610821912957257No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT13560.9603739509189418No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC13370.9469173837600481No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC12080.8555543751549276No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA12040.8527214136477921No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC11750.8321824427210596No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11520.8158929140550303No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC11460.8116434717943269No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC11310.8010198661425688No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11070.7840220970997557No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT11030.7811891355926202No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10950.7755232125783491No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC10760.7620666454194553No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC10590.7500265590141294No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10540.74648535713021No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC9650.6834519635964447No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC9450.669287156060767No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9030.6395410602358441No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8980.6359998583519246No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT8890.6296256949608697No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8770.6211268104394632No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA8690.6154608874251921No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC8660.6133361662948404No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC8130.5757994263252948No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8020.5680087821806721No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7490.5304720422111265No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC6960.4929353022415808No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6870.4865611388505259No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6860.485852898473742No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC6580.4660221679237933No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6170.4369843124756543No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6080.43061014908459927No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5670.40157229363646024No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5660.40086405325967633No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA5040.35695314989907573No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4990.35341194801515635No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4580.32437409256701727No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC4200.2974609582492298No Hit
CCTACGGGCGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA4090.2896703141046071No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3920.27763022769928114No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC3610.25567477601898086No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3520.2493006126279259No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3480.2464676511207904No Hit
CCTANGGGTGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA3390.24009348772973546No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3370.23867700697616773No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC3240.22946988207797728No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3170.22451219944049008No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3170.22451219944049008No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3020.2138885937887319No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3020.2138885937887319No Hit
CCTANGGGGGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA2940.20822267077446086No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2940.20822267077446086No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2910.20609794964410919No Hit
CCTANGGGCGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA2910.20609794964410919No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2870.2032649881369737No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2850.2018485073834059No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2840.20114026700662208No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2810.1990155458762704No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2740.19405786323878324No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2740.19405786323878324No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2710.1919331421084316No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2700.19122490173164772No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2660.1883919402245122No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2640.18697545947094443No Hit
CCTANGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA2620.1855589787173767No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2600.1841424979638089No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2570.18201777683345727No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2560.18130953645667341No Hit
CCTANGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2500.17706009419597013No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2500.17706009419597013No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA2490.17635185381918622No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2450.1735188923120507No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2440.17281065193526685No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2390.16926945005134741No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2380.16856120967456356No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2360.16714472892099577No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2250.1593540847763731No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2240.1586458443995892No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2230.15793760402280535No Hit
CCTANGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2220.15722936364602147No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2220.15722936364602147No Hit
CCTANGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2220.15722936364602147No Hit
CCTANGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA2190.15510464251566983No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT2150.15227168100853428No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA2130.15085520025496654No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2110.14943871950139878No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA2100.1487304791246149No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC2100.1487304791246149No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2080.14731399837104714No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2030.14377279648712774No Hit
CCTANGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1990.1409398349799922No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1980.14023159460320833No Hit
CCTANGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1980.14023159460320833No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA1970.13952335422642445No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1960.13881511384964057No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1960.13881511384964057No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC1950.1381068734728567No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1930.13669039271928893No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1920.13598215234250505No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC1890.1338574312121534No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1870.13244095045858564No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1790.1267750274443146No Hit
CCTANGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1720.12181734480682745No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC1720.12181734480682745No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1670.11827614292290803No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1650.11685966216934028No Hit
CCTANGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1650.11685966216934028No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1640.11615142179255639No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1640.11615142179255639No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1630.11544318141577252No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1620.11473494103898864No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1570.11119373915506924No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1570.11119373915506924No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1570.11119373915506924No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1560.11048549877828535No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1540.10906901802471759No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1500.10623605651758207No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1460.10340309501044653No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1460.10340309501044653No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT1450.10269485463366268No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1450.10269485463366268No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1440.10198661425687879No Hit
CCTANGGGAGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1420.10057013350331104No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC1420.10057013350331104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)