FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60806
Sequences flagged as poor quality0
Sequence length301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC12562.065585632996744No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC12081.986646054665658No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT11921.9603328618886293No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT11131.8304114725520508No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC10901.792586257935072No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT10521.7300924250896292No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC9931.6330625267243364No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC9011.481761668256422No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT8891.4620267736736505No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7381.2136960168404434No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5740.9439857908759003No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5460.8979377035161005No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5230.8601124888991217No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4740.7795283360194718No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4140.6808538631056146No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4090.6726309903627932No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3830.6298720521001216No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3770.620004604808736No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3740.6150708811630431No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3480.5723119429003717No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3320.5459987501233431No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3280.5394204519290859No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2730.44896885175805024No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2700.44403512811235735No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2450.4029207643982502No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2410.396342466203993No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2290.3766075716212216No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2280.3749629970726573No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2240.36838469887840014No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2230.3667401243298359No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2230.3667401243298359No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2210.36345097523270725No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2150.3535835279413216No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2050.3371377824556787No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2030.33384863335855014No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2000.3289149097128573No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1990.327270335164293No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1940.31904746242147153No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1830.3009571423872644No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1730.2845113969016216No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1700.2795776732559287No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1680.2762885241588001No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1600.2631319277702858No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1550.2549090550274644No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1510.24833075683320724No Hit
CCTACGGGGGGCAGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1460.24010788409038583No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1440.23681873499325723No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1420.23352958589612868No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1390.22859586225043582No Hit
CCTACGGGTGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1390.22859586225043582No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1320.2170838404104858No Hit
CCTACGGGCGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1310.21543926586192153No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1310.21543926586192153No Hit
CCTACGGGGGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1280.21050554221622866No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1270.2088609676676644No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1230.20228266947340726No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1190.19570437127915005No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1190.19570437127915005No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1180.1940597967305858No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT1170.1924152221820215No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1140.18748149853632864No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1120.18419234943920007No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1110.1825477748906358No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1100.1809032003420715No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1080.17761405124494292No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1070.17596947669637866No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA1060.17432490214781435No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1050.1726803275992501No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1040.17103575305068577No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA1030.16939117850212151No Hit
CCTANGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1030.16939117850212151No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC1000.16445745485642865No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1000.16445745485642865No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1000.16445745485642865No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT990.16281288030786434No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT980.16116830575930008No Hit
CCTACGGGGGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC970.15952373121073576No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC970.15952373121073576No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT950.1562345821136072No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT950.1562345821136072No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC950.1562345821136072No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT950.1562345821136072No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA940.15459000756504293No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA940.15459000756504293No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT930.15294543301647864No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT920.15130085846791436No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT920.15130085846791436No Hit
CCTACGGGGGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC900.14801170937078578No Hit
CCTACGGGTGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC890.1463671348222215No Hit
CCTACGGGCGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC880.1447225602736572No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT880.1447225602736572No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC880.1447225602736572No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC860.14143341117652863No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT860.14143341117652863No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA860.14143341117652863No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA840.13814426207940006No Hit
CCTACGGGAGGCAGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA830.13649968753083577No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT820.13485511298227149No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA820.13485511298227149No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT820.13485511298227149No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC800.1315659638851429No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC770.12663224023945005No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT770.12663224023945005No Hit
CCTANGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT770.12663224023945005No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT770.12663224023945005No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC760.12498766569088576No Hit
CCTANGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT760.12498766569088576No Hit
CCTACGGGAGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA750.12334309114232149No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT740.12169851659375719No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC740.12169851659375719No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC740.12169851659375719No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC730.12005394204519292No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC710.11676479294806434No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC710.11676479294806434No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT710.11676479294806434No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC690.11347564385093577No Hit
CCTACGGGAGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC680.11183106930237148No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA670.11018649475380718No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA670.11018649475380718No Hit
CCTACGGGTGGCTGCAGTGGGAGGACCACTTGAGCCTAGGAATTCAAGGC670.11018649475380718No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT640.10525277110811433No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA640.10525277110811433No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC620.10196362201098576No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC620.10196362201098576No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC620.10196362201098576No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT610.10031904746242147No Hit
CCTANGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT610.10031904746242147No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)