FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124192
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC66095.321598814738469No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC58684.724942025251224No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC56084.515588765782015No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC49844.013140943055913No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC42263.4027956712187577No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC31072.5017714506570474No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC15991.2875225457356352No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC13071.0524027312548312No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC11090.8929721721205874No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC10860.8744524607060036No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC9690.7802434939448596No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC9280.7472300953362535No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8640.6956969853130637No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8430.6787876835867045No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7610.6127608863694924No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7030.5660590054109765No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6950.5596173666580778No Hit
CCTACGGGTGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG6500.5233831486730224No Hit
CCTACGGGGGGCAGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG5930.4774864725586189No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5890.47426565318216957No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5510.44366786910590056No Hit
CCTACGGGCGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG5040.40582324143262044No Hit
CCTACGGGGGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG4690.3776410718886885No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4400.35429013140943055No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG4320.34784849265653184No Hit
CCTACGGGAGGCAGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG4090.329328781241948No Hit
CCTACGGGTGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG3530.2842373099716568No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3510.2826269002834321No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3440.27699046637464575No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3370.2713540324658593No Hit
CCTACGGGAGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG3280.2641071888688482No Hit
CCTACGGGGGGCAGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG3240.26088636949239885No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3160.25444473073950014No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2940.2367302241690286No Hit
CCTACGGGGGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG2920.23511981448080393No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2820.22706776603968049No Hit
CCTACGGGCGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG2690.21660010306622005No Hit
CCTANGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2660.21418448853388306No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2640.21257407884565835No Hit
CCTACGGGTGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC2610.2101584643133213No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2580.20774284978098428No Hit
CCTACGGGGGGCAGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC2320.18680752383406338No Hit
CCTANGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2270.18278149961350168No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2210.17795027054882762No Hit
CCTACGGGTGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2170.17472945117237826No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2170.17472945117237826No Hit
CCTACGGGTGGCTGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC2160.17392424632826592No Hit
CCTACGGGGGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC2120.17070342695181653No Hit
CCTACGGGGGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2070.16667740273125484No Hit
CCTACGGGAGGCAGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG2040.1642617881989178No Hit
CCTACGGGCGGCTGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC1920.1545993300695697No Hit
CCTACGGGCGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1920.1545993300695697No Hit
CCTACGGGGGGCAGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC1920.1545993300695697No Hit
CCTACGGGGGGCTGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC1860.14976810100489565No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1850.14896289616078331No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1850.14896289616078331No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1800.1449368719402216No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1760.1417160525637722No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1700.13688482349909817No Hit
CCTACGGGTGGCAGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1680.1352744138108735No Hit
CCTACGGGTGGCTGCAGGCAGCAGGCAGCGCGCGGTACTGGCGAGTTCTC1680.1352744138108735No Hit
CCTACGGGGGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1660.1336640041226488No Hit
CCTACGGGAGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1650.13285879927853644No Hit
CCTACGGGTGGCTGCAGGAGGCAGAGAGGAAGCTGGCAGTTGCTGCACAC1630.13124838959031176No Hit
CCTANGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1590.1280275702138624No Hit
CCTACGGGGGGCAGCAGGAGGCAGAGAGGAAGCTGGCAGTTGCTGCACAC1550.12480675083741304No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1540.1240015459933007No Hit
CCTANGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1500.12078072661685132No Hit
CCTACGGGCGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1490.11997552177273897No Hit
CCTACGGGGGGCAGCAGGCAGCAGGCAGCGCGCGGTACTGGCGAGTTCTC1480.11917031692862663No Hit
CCTACGGGTGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1430.11514429270806494No Hit
CCTACGGGAGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1390.11192347333161556No Hit
CCTACGGGAGGCAGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1370.11031306364339087No Hit
CCTACGGGCGGCAGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1350.10870265395516619No Hit
CCTACGGGGGGCAGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1340.10789744911105384No Hit
CCTACGGGAGGCAGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC1310.10548183457871683No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1300.1046766297346045No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1290.10387142489049214No Hit
CCTACGGGAGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1280.1030662200463798No Hit
CCTACGGGGGGCTGCAGGCAGCAGGCAGCGCGCGGTACTGGCGAGTTCTC1270.10226101520226746No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)