FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124192
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA71515.758019840247359No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA50724.0839989693378No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA48633.91571115691832No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA42013.3826655501159495No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA37082.985699561968565No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA31672.5500837413037876No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA26012.094337799536202No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA24261.953426951816542No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA14461.1643262045864469No Hit
GACTACAAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG6790.5467340891522804No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4590.36958902344756506No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4580.3687838186034527No Hit
GACTACACGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG4580.3687838186034527No Hit
GACTACAGGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG4290.34543287812419476No Hit
GACTACAAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT3920.31564029889203815No Hit
GACTACTAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG3920.31564029889203815No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3670.29551017778922956No Hit
GACTACTCGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG3520.2834321051275444No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3440.27699046637464575No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3370.2713540324658593No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3240.26088636949239885No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3220.2592759598041742No Hit
GACTACCCGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG2970.23914583870136563No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2860.23028858541612987No Hit
GACTACAAGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC2770.2230417418191188No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2740.22062612728678174No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2740.22062612728678174No Hit
GACTACTGGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG2570.20693764493687192No Hit
GACTACAAGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC2510.2021064158721979No Hit
GACTACACGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT2380.19163875289873744No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2380.19163875289873744No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2360.19002834321051276No Hit
GANTACAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2350.1892231383664004No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2320.18680752383406338No Hit
GACTACCAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG2290.18439190930172636No Hit
GANTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2290.18439190930172636No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2240.18036588508116463No Hit
GANTANAAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2220.17875547539293996No Hit
GACTANAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2210.17795027054882762No Hit
GACTACAGGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT2110.1698982221077042No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2110.1698982221077042No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2100.16909301726359185No Hit
GACTACAAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA2050.16506699304303013No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2040.1642617881989178No Hit
GACTACTAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT2000.16104096882246843No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1940.15620973975779437No Hit
GACTACTCGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1940.15620973975779437No Hit
GACTACAGGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1910.15379412522545735No Hit
GACTACACGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1880.15137851069312033No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1870.150573305849008No Hit
GANTANACGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1840.14815769131667098No Hit
GACTACCCGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1730.13930043803143521No Hit
GACTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1720.13849523318732287No Hit
GANTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1710.1376900283432105No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1700.13688482349909817No Hit
GANTANAGGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1690.13607961865498583No Hit
GACTACAGGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1690.13607961865498583No Hit
GANTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1640.1320535944344241No Hit
GANTACAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1620.13044318474619943No Hit
GACTACTAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1620.13044318474619943No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1600.12883277505797475No Hit
GANTACACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1600.12883277505797475No Hit
GACTACAGGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1590.1280275702138624No Hit
GACTACTAGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1580.12722236536975007No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1580.12722236536975007No Hit
GACTACACGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1560.1256119556815254No Hit
GACTACAAGGGTATCTAATCCATTATTTGAAGCGGGCTCGGAGGAAACGT1560.1256119556815254No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1560.1256119556815254No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1550.12480675083741304No Hit
GACTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1540.1240015459933007No Hit
GACTACACGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1530.12319634114918836No Hit
GACTACTGGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1520.122391136305076No Hit
GACTANAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1430.11514429270806494No Hit
GANTACTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1390.11192347333161556No Hit
GACTACTAGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1390.11192347333161556No Hit
GACTACCAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1390.11192347333161556No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1380.11111826848750322No Hit
GACTACAAGGGTATCTAATCCCAGACTGTTTTCTGGCCTTGAGAATCTTA1380.11111826848750322No Hit
GACTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1380.11111826848750322No Hit
GACTANACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1360.10950785879927853No Hit
GACTACCGGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1350.10870265395516619No Hit
GACTANTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1340.10789744911105384No Hit
GACTANTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1330.10709224426694153No Hit
GACTACTCGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1320.10628703942282917No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1320.10628703942282917No Hit
GANTANTAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1300.1046766297346045No Hit
GANTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1290.10387142489049214No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1290.10387142489049214No Hit
GANTACTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1270.10226101520226746No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT1250.10065060551404277No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)