FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96401
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC23502.437734048401987No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC21882.2696859991078933No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC20012.0757046088733517No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC18581.927365898693997No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC14141.466789763591664No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC11211.162851007769629No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC10661.1057976577006463No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC10231.061192311283078No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC9440.9792429539112665No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8340.8651362537733012No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7290.7562162218234251No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7150.7416935508967749No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6460.670117529901142No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6230.6462588562359312No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5720.59335484071742No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5660.5871308388917127No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5420.562234831588884No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4430.45953880146471515No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4250.4408667959875935No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3800.3941867822947895No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3790.3931494486571716No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3680.3817387786433751No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3610.37447744318005No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3540.3672161077167249No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3510.36410410680387134No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3390.3516561031524569No Hit
CCTACGGGTGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG3300.3423201004138961No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2960.30705075673488863No Hit
CCTACGGGTGGCTGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC2960.30705075673488863No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2850.2956400867210921No Hit
CCTACGGGTGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT2790.2894160848953849No Hit
CCTACGGGGGGCTGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC2780.2883787512577671No Hit
CCTACGGGGGGCAGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC2770.28734141762014914No Hit
CCTACGGGGGGCAGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG2550.26452007759255614No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2550.26452007759255614No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2510.26037074304208463No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2470.2562214084916132No Hit
CCTACGGGGGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT2400.2489600730282881No Hit
CCTACGGGGGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG2390.2479227393906702No Hit
CCTACGGGGGGCAGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT2370.2458480721154345No Hit
CCTACGGGCGGCTGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC2300.23858673665210942No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2070.21472806298689848No Hit
CCTACGGGCGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG2060.2136907293492806No Hit
CCTANGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2060.2136907293492806No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2050.21265339571166275No Hit
CCTACGGGTGGCTGCAGTTAAAGCCATCTCTGAGATTTGTGGTCATAAAT2040.21161606207404488No Hit
CCTACGGGCGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT2040.21161606207404488No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2000.2074667275235734No Hit
CCTACGGGAGGCAGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC1960.20331739297310192No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1940.20124272569786622No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1870.19398139023454114No Hit
CCTANGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1790.1856827211335982No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1790.1856827211335982No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1760.1825707202207446No Hit
CCTACGGGGGGCAGCAGTTAAAGCCATCTCTGAGATTTGTGGTCATAAAT1740.18049605294550886No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1730.17945871930789098No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1700.1763467183950374No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1690.17530938475741953No Hit
CCTANGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1690.17530938475741953No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1670.1732347174821838No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1650.17116005020694805No Hit
CCTACGGGGGGCTGCAGTTAAAGCCATCTCTGAGATTTGTGGTCATAAAT1640.1701227165693302No Hit
CCTACGGGCGGCTGCAGTTAAAGCCATCTCTGAGATTTGTGGTCATAAAT1610.1670107156564766No Hit
CCTACGGGAGGCAGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1610.1670107156564766No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1540.15974938019315152No Hit
CCTACGGGAGGCTGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC1520.1576747129179158No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1520.1576747129179158No Hit
CCTACGGGAGGCAGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1500.15560004564268004No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1460.1514507110922086No Hit
CCTANGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1440.14937604381697286No Hit
CCTACGGGAGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1400.14522670926650139No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1400.14522670926650139No Hit
CCTACGGGTGGCTGCAGTGAGCTGAGATCGTGTCACTGCACTCCTGCCTG1390.14418937562888354No Hit
CCTACGGGTGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1320.13692804016555846No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1320.13692804016555846No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1320.13692804016555846No Hit
CCTACGGGAGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1270.1317413719774691No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1260.13070403833985125No Hit
CCTACGGGAGGCAGCAGTTAAAGCCATCTCTGAGATTTGTGGTCATAAAT1250.12966670470223338No Hit
CCTACGGGGGGCTGCAGTGTGGGTTCTCATGGAAATCTTGCATTGGAGGA1220.12655470378937977No Hit
CCTACGGGTGGCTGCAGTGTGGGTTCTCATGGAAATCTTGCATTGGAGGA1220.12655470378937977No Hit
CCTACGGGGGGCAGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1220.12655470378937977No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1200.12448003651414405No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1160.12033070196367257No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1150.11929336832605471No Hit
CCTACGGGGGGCAGCAGTGAGCTGAGATCGTGTCACTGCACTCCTGCCTG1120.1161813674132011No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1100.11410670013796537No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1080.11203203286272963No Hit
CCTACGGGGGGCTGCAGTGAGCTGAGATCGTGTCACTGCACTCCTGCCTG1070.11099469922511178No Hit
CCTACGGGCGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1050.10892003194987604No Hit
CCTANGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1050.10892003194987604No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1030.1068453646746403No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT990.10269603012416885No Hit
CCTACGGGTGGCTGCAGCTGCTTTCCCAGCCTTGGATAGACAGAAAGAGG980.10165869648655096No Hit
CCTACGGGGGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA970.10062136284893311No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)