FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96401
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA30263.1389715874316657No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA21652.245827325442682No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA19201.9916805842263048No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA17831.849565875872657No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA15851.6441738156243193No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA13041.3526830634536986No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA11041.145216335930125No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA10591.0985363222373212No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7810.8101575709795541No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA6310.6545575253368741No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5580.5788321697907698No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5460.5663841661393554No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA5020.5207414860841693No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4640.4813228078546903No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3960.4107841204966754No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3890.4035227850333503No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3840.3983361168452609No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3790.3931494486571716No Hit
GACTACAAGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC3610.37447744318005No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3430.3558054377029284No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3350.3475067686019855No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3210.3329840976753353No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3180.32987209676248175No Hit
GACTACAAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG2910.3018640885467993No Hit
GACTACAAGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT2880.29875208763394573No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2770.28734141762014914No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2700.2800800821568241No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2630.27281874669349904No Hit
GACTACAGGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC2540.2634827439549382No Hit
GACTACACGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC2440.25310940757875955No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2370.2458480721154345No Hit
GACTACTAGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC2240.2323627348264022No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2200.22821340027593073No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2170.22510139936307716No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2130.22095206481260568No Hit
GACTACAGGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT2070.21472806298689848No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2030.210578728436427No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2030.210578728436427No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2030.210578728436427No Hit
GACTACACGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT2030.210578728436427No Hit
GACTACAGGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1870.19398139023454114No Hit
GACTACAAGGGTATCTAATCCGCTAAACAGAGGTAGCAAAAAAAATACAA1850.1919067229593054No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1820.1887947220464518No Hit
GACTACACGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1800.18672005477121606No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1760.1825707202207446No Hit
GACTACTAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1760.1825707202207446No Hit
GACTACTCGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1760.1825707202207446No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1760.1825707202207446No Hit
GACTACCCGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1730.17945871930789098No Hit
GACTACTAGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1730.17945871930789098No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1710.17738405203265528No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1640.1701227165693302No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1590.16493604838124087No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1570.16286138110600512No Hit
GACTACTCGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1510.15663737928029794No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1500.15560004564268004No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1490.1545627120050622No Hit
GACTACTCGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1450.1504133774545907No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1440.14937604381697286No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1420.1473013765417371No Hit
GACTACTGGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1380.14315204199126563No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1360.14107737471602994No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1350.14004004107841206No Hit
GACTACAAGGGTATCTAATCCTTTCAGTAGGGAGACACACATGGTCAAGG1330.1379653738031763No Hit
GACTACACGGGTATCTAATCCGCTAAACAGAGGTAGCAAAAAAAATACAA1290.13381603925270485No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1270.1317413719774691No Hit
GACTACCCGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1270.1317413719774691No Hit
GACTACCCGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1270.1317413719774691No Hit
GACTACAGGGGTATCTAATCCGCTAAACAGAGGTAGCAAAAAAAATACAA1250.12966670470223338No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1230.12759203742699765No Hit
GACTACAAGGGTATCTAATCCCACCTAGAAGCCCACTTGACAGTTTTGTT1180.12240536923890831No Hit
GACTACCAGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1170.12136803560129045No Hit
GACTACTAGGGTATCTAATCCGCTAAACAGAGGTAGCAAAAAAAATACAA1160.12033070196367257No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1150.11929336832605471No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1150.11929336832605471No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1140.11825603468843685No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1110.11514403377558323No Hit
GACTACTGGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1080.11203203286272963No Hit
GACTACTGGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1060.10995736558749389No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1060.10995736558749389No Hit
GACTACAAGGGTATCTAATCCTAAAACAACCAAAGTCTCGCCACCATGGC1020.10580803103702244No Hit
GANTACAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1020.10580803103702244No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1020.10580803103702244No Hit
GACTACAAGGGTATCTAATCCTGGGAAACTCACCTCTGAACAGAAGCCCA1010.10477069739940456No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG990.10269603012416885No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG980.10165869648655096No Hit
GACTACAAGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC970.10062136284893311No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)