FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125150
Sequences flagged as poor quality0
Sequence length301
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC79756.372353176188573No Hit
CCTACGGGTGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC79006.3124250898921295No Hit
CCTACGGGGGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC68005.433479824210947No Hit
CCTACGGGCGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC62585.00039952057531No Hit
CCTACGGGAGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC57264.575309628445865No Hit
CCTACGGGAGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC39123.125848981222533No Hit
CCTACGGGTGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC23071.8433879344786255No Hit
CCTACGGGCGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC18211.4550539352776668No Hit
CCTANGGGTGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC16591.325609268877347No Hit
CCTANGGGGGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC16551.3224131042748701No Hit
CCTANGGGGGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC13951.1146624051138634No Hit
CCTANGGGCGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC13491.0779065121853777No Hit
CCTANGGGAGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC11860.9476628046344387No Hit
CCTANGGGAGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC7600.6072712744706352No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5020.40111865761086696No Hit
CCTACGGGTGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC4760.3803435876947663No Hit
CCTANGGGTGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC4560.36436276468238116No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4330.3459848182181382No Hit
CCTACGGGGGGCAGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC4170.33320015980823015No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4000.31961646024770274No Hit
CCTANGGGCGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC3780.30203755493407913No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3750.2996404314822213No Hit
CCTACGGGTGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC3710.2964442668797443No Hit
CCTACGGGCGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC3610.2884538553735517No Hit
CCTACGGGGGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC3600.2876548142229325No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG3260.26048741510187773No Hit
CCTACGGGGGGCAGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC3190.254894127047543No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2930.23411905713144227No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2910.23252097483020376No Hit
CCTACGGGAGGCAGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC2880.23012385137834598No Hit
CCTACGGGGGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC2660.21254494606472232No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2570.20535357570914903No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2530.202157411106672No Hit
CCTACGGGCGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC2480.1981622053535757No Hit
CCTACGGGAGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC2390.19097083499800238No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2390.19097083499800238No Hit
CCTACGGGAGGCAGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC2360.18857371154614463No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2340.1869756292449061No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2260.18058330003995207No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2210.1765880942868558No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2180.17419097083499802No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2150.17179384738314024No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2080.16620055932880543No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1860.1486216540151818No Hit
CCTACGGGTGGCTGCAGCCGCATCTTCTTCCATCCTCAAGGCTGTTGTGG1830.14622453056332402No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1820.14542548941270475No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1800.14382740711146624No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGGAGGAATCCTGAT1750.13983220135836996No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGCTAATCG1690.1350379544546544No Hit
CCTACGGGGGGCAGCAGCCGTGGTAATACAGAGGGTGCGAGCGTTAATCG1660.13264083100279664No Hit
CCTACGGGGGGCAGCAGCCGCATCTTCTTCCATCCTCAAGGCTGTTGTGG1640.13104274870155813No Hit
CCTACGGGTGGCTGCAGCCGTGGTAATACAGAGGGTGCGAGCGTTAATCG1630.13024370755093886No Hit
CCTACGGGGGGCTGCAGCCGCATCTTCTTCCATCCTCAAGGCTGTTGTGG1620.12944466640031962No Hit
CCTACGGGAGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC1620.12944466640031962No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1600.1278465840990811No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1590.12704754294846185No Hit
CCTACGGGGGGCAGCAGCCGCGGTAAGACAGAGGATGCAAGCGTTATCCG1560.12465041949660408No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1560.12465041949660408No Hit
CCTACGGGTGGCTGCAGCCGCGGTAAGACAGAGGATGCAAGCGTTATCCG1540.12305233719536557No Hit
CCTACGGGCGGCTGCAGCCGCATCTTCTTCCATCCTCAAGGCTGTTGTGG1510.1206552137435078No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1470.11745904914103077No Hit
CCTACGGGTGGCTGCAGTTGCAACGGAGGAATTCTGTGGTGTTCCCGGTT1410.11266480223731522No Hit
CCTACGGGCGGCTGCAGCCGCGGTAAGACAGAGGATGCAAGCGTTATCCG1400.11186576108669595No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1360.10866959648421894No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1340.10707151418298043No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1340.10707151418298043No Hit
CCTACGGGCGGCTGCAGTTGCAACGGAGGAATTCTGTGGTGTTCCCGGTT1340.10707151418298043No Hit
CCTACGGGTGGCAGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC1340.10707151418298043No Hit
CCTACGGGAGGCAGCAGCCGCATCTTCTTCCATCCTCAAGGCTGTTGTGG1330.10627247303236116No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1310.10467439073112265No Hit
CCTACGGGGGGCTGCAGCCGCGGTAAGACAGAGGATGCAAGCGTTATCCG1300.10387534958050339No Hit
CCTACGGGGGGCTGCAGTTGCAACGGAGGAATTCTGTGGTGTTCCCGGTT1290.10307630842988415No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGCTAATCG1280.10227726727926488No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGCTAATCG1270.10147822612864563No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1270.10147822612864563No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)