FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125150
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT93957.506991610067919No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT62755.013983220135837No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT59644.765481422293248No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT56364.503395924890132No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT49863.9840191769876148No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT42473.3935277666799837No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT34832.7830603276068717No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT33082.643228126248502No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT20731.6564123052337194No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG8190.6544147023571714No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6900.5513383939272872No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG5240.4186975629244906No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG5210.41630043947263284No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG4660.3723531761885737No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4470.35717139432680783No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4210.33639632441070716No Hit
GACTACAAGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG4170.33320015980823015No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4140.3308030363563723No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG3950.3156212544946065No Hit
GANTANAAGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT3870.3092289252896524No Hit
GACTANAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT3660.29244906112664804No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG3610.2884538553735517No Hit
GANTACAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT3570.2852576907710747No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3550.2836596084698362No Hit
GACTACAGGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG3490.27886536156612063No Hit
GANTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT3480.2780663204155014No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT3230.25809029165002No Hit
GACTACACGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG3220.2572912504994007No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3040.24290850978825412No Hit
GACTACTAGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG2930.23411905713144227No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2820.22532960447463043No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2790.2229324810227727No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2780.22213343987215342No Hit
GANTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2780.22213343987215342No Hit
GACTACAAGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT2700.21574111066719934No Hit
GANTANAGGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2630.21014782261286458No Hit
GACTANACGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2590.20695165801038756No Hit
GACTACTCGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG2580.2061526168597683No Hit
GANTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2510.20055932880543348No Hit
GACTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2500.1997602876548142No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2490.19896124650419497No Hit
GACTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2450.19576508190171793No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2430.19416699960047942No Hit
GACTANAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2400.19176987614862165No Hit
GANTANACGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2390.19097083499800238No Hit
GANTACAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2340.1869756292449061No Hit
GANTACACGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2320.1853775469436676No Hit
GACTACTAGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT2310.18457850579304835No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2270.1813823411905713No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2230.1781861765880943No Hit
GANTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2170.17339192968437875No Hit
GACTANTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2130.1701957650819017No Hit
GACTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2080.16620055932880543No Hit
GACTACCCGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG2030.16220535357570914No Hit
GACTACAAGGGTATCTAATCCTAAGCTTGAACGTGAGAGCGAAGCTCCAT2030.16220535357570914No Hit
GACTACAGGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT2010.16060727127447064No Hit
GANTANTCGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1950.1558130243707551No Hit
GANTACTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1940.15501398322013585No Hit
GANTANTAGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1930.15421494206951658No Hit
GANTANTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1920.1534159009188973No Hit
GACTACAAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCCCAG1910.15261685976827807No Hit
GACTANTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1840.14702357171394329No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1830.14622453056332402No Hit
GACTANTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1800.14382740711146624No Hit
GANTACTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1780.14222932481022774No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1770.14143028365960847No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1750.13983220135836996No Hit
GACTACTGGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG1730.13823411905713145No Hit
GACTACACGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT1650.13184178985217737No Hit
GACTACAAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGCGCCTCAG1650.13184178985217737No Hit
GACTANCCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1630.13024370755093886No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1620.12944466640031962No Hit
GANTANCCGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1590.12704754294846185No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1580.12624850179784258No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1560.12465041949660408No Hit
GACTACAGGGGTATCTAATCCTAAGCTTGAACGTGAGAGCGAAGCTCCAT1550.12385137834598481No Hit
GACTACAAGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA1520.12145425489412706No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1510.1206552137435078No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1510.1206552137435078No Hit
GACTANCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1510.1206552137435078No Hit
GANTANCCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1500.11985617259288853No Hit
GACTACACGGGTATCTAATCCTAAGCTTGAACGTGAGAGCGAAGCTCCAT1500.11985617259288853No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1480.11825809029165002No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1480.11825809029165002No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1470.11745904914103077No Hit
GACTACCAGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG1450.11586096683979225No Hit
GANTACCCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1450.11586096683979225No Hit
GANTANTGGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1420.11346384338793448No Hit
GANTANCAGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1420.11346384338793448No Hit
GACTANTGGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1390.11106671993607671No Hit
GANTANTGGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1390.11106671993607671No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1370.1094686376348382No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG1340.10707151418298043No Hit
GACTANTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1310.10467439073112265No Hit
GACTACTCGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT1300.10387534958050339No Hit
GACTACACGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA1290.10307630842988415No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCCCAG1270.10147822612864563No Hit
GANTANCAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1270.10147822612864563No Hit
GACTACTGGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT1270.10147822612864563No Hit
GACTACAGGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA1270.10147822612864563No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1260.10067918497802637No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)