FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71083
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC28794.050194842648735No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC25563.595796463289394No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC23773.3439781663688923No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC23293.276451472222613No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC19662.765780847741373No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC14562.048309722437151No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7691.0818339124685228No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6460.908796758718681No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6210.8736266055174937No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6130.8623721564931137No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5940.8356428400602113No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5870.8257951971638788No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5760.8103203297553565No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5660.7962522684748815No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5220.7343527988407917No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5130.7216915436883643No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4860.683707778231082No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3930.552874808322665No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3530.49660256320076535No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2900.40797377713377325No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2460.3460743074996835No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG2350.33059944009116105No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG2160.30387012365825866No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2120.29824289914606866No Hit
CCTACGGGTGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG2040.2869884501216888No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1940.2729203888412138No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1910.26869997045707134No Hit
CCTACGGGGGGCAGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1900.26729316432902384No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1880.26447955207292884No Hit
CCTACGGGTGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1800.2532251030485489No Hit
CCTACGGGGGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1760.2475978785363589No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1750.2461910724083114No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1710.24056384789612142No Hit
CCTACGGGGGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1620.22790259274369398No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1560.219461755975409No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1540.216648143719314No Hit
CCTANGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1520.21383453146321904No Hit
CCTACGGGCGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1500.21102091920712404No Hit
CCTACGGGGGGCAGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1490.20961411307907654No Hit
CCTANGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1480.20820730695102907No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1450.20398688856688663No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1450.20398688856688663No Hit
CCTACGGGGGGCAGCAGATGCTCCAGAGTTTGTGGATATGAAGAGTGTGG1440.20258008243883913No Hit
CCTANGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1410.19835966405469663No Hit
CCTACGGGCGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1370.19273243954250663No Hit
CCTANGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1360.19132563341445916No Hit
CCTACGGGAGGCAGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1340.18851202115836416No Hit
CCTACGGGAGGCAGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1320.18569840890226919No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1320.18569840890226919No Hit
CCTACGGGTGGCTGCAGATGCTCCAGAGTTTGTGGATATGAAGAGTGTGG1270.17866437826203171No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1270.17866437826203171No Hit
CCTACGGGTGGCTGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC1230.17303715374984174No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1220.17163034762179424No Hit
CCTACGGGCGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1210.17022354149374674No Hit
CCTANGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1210.17022354149374674No Hit
CCTACGGGTGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1180.16600312310960427No Hit
CCTACGGGGGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1170.16459631698155677No Hit
CCTACGGGTGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1160.16318951085350927No Hit
CCTANGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1150.16178270472546177No Hit
CCTACGGGTGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1140.1603758985974143No Hit
CCTACGGGGGGCAGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1100.1547486740852243No Hit
CCTACGGGGGGCAGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1100.1547486740852243No Hit
CCTACGGGGGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1100.1547486740852243No Hit
CCTACGGGGGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1050.14771464344498683No Hit
CCTACGGGAGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1040.14630783731693933No Hit
CCTACGGGGGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1020.1434942250608444No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1020.1434942250608444No Hit
CCTACGGGGGGCTGCAGATGCTCCAGAGTTTGTGGATATGAAGAGTGTGG1020.1434942250608444No Hit
CCTACGGGCGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1020.1434942250608444No Hit
CCTANGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC990.1392738066767019No Hit
CCTACGGGAGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG950.13364658216451192No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG930.13083296990841692No Hit
CCTACGGGAGGCAGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG920.12942616378036942No Hit
CCTACGGGGGGCAGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC920.12942616378036942No Hit
CCTACGGGAGGCAGCAGATGCTCCAGAGTTTGTGGATATGAAGAGTGTGG910.12801935765232195No Hit
CCTACGGGCGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC900.12661255152427445No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG880.12379893926817945No Hit
CCTACGGGCGGCTGCAGATGCTCCAGAGTTTGTGGATATGAAGAGTGTGG850.11957852088403696No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG850.11957852088403696No Hit
CCTACGGGAGGCAGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC830.11676490862794198No Hit
CCTACGGGAGGCAGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC830.11676490862794198No Hit
CCTACGGGGGGCTGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC820.11535810249989449No Hit
CCTACGGGAGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC790.111137684115752No Hit
CCTANGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC750.10551045960356202No Hit
CCTACGGGGGGCAGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA720.10129004121941956No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)