FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71083
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA32574.581967559050687No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA22453.1582797574666235No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA20802.926156746338787No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA19352.7221698577719002No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA17222.4225201524977846No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA14852.089107100150528No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA12301.7303715374984172No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA10801.5193506182912933No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA7441.0466637592673353No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6390.8989491158223485No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA5160.7259119620725069No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4560.6415035943896572No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4230.5950789921640899No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4110.57819731862752No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3750.5275522980178101No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3690.5191114612495251No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3550.49941617545686023No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3080.433296287438628No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2980.4192282261581531No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2680.3770240423167283No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2350.33059944009116105No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2330.32778582783506605No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2320.32637902170701855No Hit
GACTACAAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT2290.32215860332287605No Hit
GACTACAAGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT2160.30387012365825866No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2070.29120886850583116No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1820.2560387153046439No Hit
GACTACAAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1710.24056384789612142No Hit
GACTACAAGGGTATCTAATCCTCTGTCACATACCTCATCTCAACAGCCAA1620.22790259274369398No Hit
GACTACACGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1570.2208685621034565No Hit
GACTACAAGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1520.21383453146321904No Hit
GACTACAGGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1500.21102091920712404No Hit
GANTANAAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1470.2068005008229816No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1410.19835966405469663No Hit
GACTACAAGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1370.19273243954250663No Hit
GACTACACGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1360.19132563341445916No Hit
GACTACAGGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1350.18991882728641166No Hit
GACTANAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1310.18429160277422169No Hit
GANTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1290.18147799051812671No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1290.18147799051812671No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1280.18007118439007921No Hit
GACTACTCGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1240.17444395987788924No Hit
GACTACTAGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1220.17163034762179424No Hit
GACTACTAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1190.16740992923765177No Hit
GANTACAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1150.16178270472546177No Hit
GACTACTCGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1120.1575622863413193No Hit
GACTACAGGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1040.14630783731693933No Hit
GACTACTAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1010.1420874189327969No Hit
GACTACAAGGGTATCTAATCCCAGTAAAAATTTCTTTTTAATAGAAATTA1010.1420874189327969No Hit
GANTACAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1000.1406806128047494No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG990.1392738066767019No Hit
GACTACACGGGTATCTAATCCTCTGTCACATACCTCATCTCAACAGCCAA990.1392738066767019No Hit
GACTACAGGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC980.1378670005486544No Hit
GANTANACGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA970.1364601944206069No Hit
GANTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA940.13223977603646442No Hit
GACTACACGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG940.13223977603646442No Hit
GACTACAGGGGTATCTAATCCTCTGTCACATACCTCATCTCAACAGCCAA930.13083296990841692No Hit
GACTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA920.12942616378036942No Hit
GACTACTGGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT910.12801935765232195No Hit
GACTACACGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC880.12379893926817945No Hit
GACTACCCGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT870.12239213314013196No Hit
GACTACTAGGGTATCTAATCCTCTGTCACATACCTCATCTCAACAGCCAA870.12239213314013196No Hit
GACTACAAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA870.12239213314013196No Hit
GANTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA870.12239213314013196No Hit
GACTACTAGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC860.12098532701208446No Hit
GACTACTAGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC860.12098532701208446No Hit
GACTACACGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC860.12098532701208446No Hit
GACTANACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA850.11957852088403696No Hit
GACTACCCGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT840.11817171475598948No Hit
GACTACAGGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA830.11676490862794198No Hit
GACTACAGGGGTATCTAATCCCAGTAAAAATTTCTTTTTAATAGAAATTA830.11676490862794198No Hit
GACTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA830.11676490862794198No Hit
GANTANTAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA820.11535810249989449No Hit
GANTANTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA810.11395129637184699No Hit
GANTACTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA810.11395129637184699No Hit
GACTACTAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA800.11254449024379949No Hit
GACTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA800.11254449024379949No Hit
GACTACAGGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG790.111137684115752No Hit
GANTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA790.111137684115752No Hit
GANTACACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA790.111137684115752No Hit
GANTANAGGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA780.1097308779877045No Hit
GACTACCAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT780.1097308779877045No Hit
GACTACTGGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT770.108324071859657No Hit
GACTANAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA760.10691726573160952No Hit
GACTANTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA750.10551045960356202No Hit
GACTACAAGGGTATCTAATCCATTATTTGAAGCGGGCTCGGAGGAAACGT740.10410365347551453No Hit
GACTANTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA730.10269684734746706No Hit
GANTANTCGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA720.10129004121941956No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG720.10129004121941956No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG720.10129004121941956No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)