FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110946
Sequences flagged as poor quality0
Sequence length301
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT34003.064553927135724No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT29282.6391217348980587No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT26542.3921547419465323No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC26032.3461864330394966No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC25562.303823481693797No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT24292.1893533791213744No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT23542.1217529248463216No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC22502.0280136282515815No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT21741.9595118345861953No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT21021.8946153984821443No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC20761.8711805743334597No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT19391.7476970778576966No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC18321.6512537630919546No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT18301.6494510843112864No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT15541.4006814125790925No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT14801.3339822976943738No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC12831.1564184377985687No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT11431.0302309231518036No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT8710.7850666089809457No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7130.6426549853081679No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6400.5768572098137833No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6380.5750545310331152No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6300.567843815910443No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6080.5480143493230941No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6000.5408036342004218No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5580.5029473798063923No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5370.48401925260937756No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5090.4587817496800245No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5050.45517639211868843No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4950.446162998215348No Hit
CCTACGGGTGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT4890.4407549618733438No Hit
CCTACGGGGGGCAGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT4760.42903754979900127No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4720.42543219223766515No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4660.42002415589566094No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4640.41822147711499286No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4530.4083067438213185No Hit
CCTACGGGTGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA4440.40019468930831215No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4440.40019468930831215No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4390.3956879923566419No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4240.38216790150163144No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4120.37135182881762296No Hit
CCTACGGGGGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA3920.35332504101094225No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3760.33890361076559766No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3710.3343969138139275No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3700.33349557442359345No Hit
CCTACGGGGGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT3630.3271861986912552No Hit
CCTACGGGCGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT3470.3127647684459106No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3410.3073567321039064No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3320.29924467759090007No Hit
CCTACGGGAGGCAGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT3240.29203396246822777No Hit
CCTACGGGGGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA3170.2857245867358895No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3060.2758098534422151No Hit
CCTACGGGCGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA2990.26950047770987684No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2930.26409244136787263No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2900.26138842319687056No Hit
CCTACGGGAGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA2660.23975627782885367No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2610.2352495808771835No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2530.2280388657545112No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2510.22623618697384315No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2480.223532168802841No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2350.21181475672849853No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2350.21181475672849853No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2340.21091341733816454No Hit
CCTACGGGAGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT2180.19649198709281993No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2100.18928127197014763No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1960.17666252050547113No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1890.17035314477313288No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1870.16855046599246482No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1800.16224109026012656No Hit
CCTACGGGAGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA1800.16224109026012656No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1770.15953707208912443No Hit
CCTACGGGTGGCTGCAGTCGCGAAAACTTCACACTGGGGGCAACCCCGAT1700.15322769635678618No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1620.14601698123411389No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1570.14151028428244372No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1530.13790492672110755No Hit
CCTANGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1510.1361022479404395No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1490.13429956915977143No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1450.13069421159843528No Hit
CCTACGGGGGGCAGCAGTCGCGAAAACTTCACACTGGGGGCAACCCCGAT1440.12979287220810123No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1430.1288915328177672No Hit
CCTANGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1390.12528617525643107No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1370.12348349647576298No Hit
CCTANGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1290.11627278135309069No Hit
CCTACGGGTGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1290.11627278135309069No Hit
CCTACGGGGGGCTGCAGTCGCGAAAACTTCACACTGGGGGCAACCCCGAT1280.11537144196275666No Hit
CCTACGGGTGGCAGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT1280.11537144196275666No Hit
CCTACGGGTGGCTGNAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1270.11447010257242263No Hit
CCTACGGGTGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1260.11356876318208858No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1250.11266742379175455No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1210.10906206623041839No Hit
CCTACGGGCGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1190.10725938744975032No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTATGTTCCAAGCGTTATCCG1190.10725938744975032No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1180.10635804805941629No Hit
CCTACGGGCGGCTGCAGTCGCGAAAACTTCACACTGGGGGCAACCCCGAT1150.10365402988841418No Hit
CCTACGGGGGGCTGNAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1130.10185135110774611No Hit
CCTACGGGTGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA1110.10004867232707804No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)