FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004833476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9491
Sequences flagged as poor quality0
Sequence length35-251
%GC58

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTTGTAGTC1251.317037193130334No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTTGTAGTC1221.285428300495206No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTTGTAGTC1081.1379201348646086No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTTGTAGTC860.9061215888736698No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGTGTAGTC820.8639763986934991No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTAGTAGTC710.7480771256980298No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTTGTAGTC700.737540828152987No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTTGTAGTC680.7164682330629016No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCCTGTAGTC670.705931935517859No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCCTGTAGTC660.6953956379728163No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGTGTAGTC650.6848593404277737No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGAGTAGTC640.6743230428827309No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTAGTAGTC640.6743230428827309No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTAGTAGTC630.6637867453376883No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGTGTAGTC610.642714150247603No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGGGTAGTC590.6216415551575176No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGAGTAGTC590.6216415551575176No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTTGTAGTC580.611105257612475No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTTGTAGTC570.6005689600674323No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGGGTAGTC560.5900326625223896No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGTGTAGTC550.5794963649773469No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCCTGTAGTC550.5794963649773469No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG500.5268148772521336No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTTGTAGTC500.5268148772521336No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGAGTAGTC480.5057422821620482No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTTGTAGTC480.5057422821620482No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGGGTAGTC470.4952059846170056No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTGGTAGTC430.4530607944368349No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCCTGTAGTC430.4530607944368349No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGTGTAGTC420.44252449689179224No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGAGTAGTC410.43198819934674954No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCCAGTAGTC400.4214519018017069No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCCTGTAGTC390.41091560425666424No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTGGTAGTC380.4003793067116216No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGGGTAGTC370.3898430091665789No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGTGTAGTC360.3793067116215362No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTTGTAGTC360.3793067116215362No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCTTGTAGTC350.3687704140764935No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCCAGTAGTC350.3687704140764935No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCTTGTAGTC350.3687704140764935No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGAGTAGTC350.3687704140764935No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTGGTAGTC350.3687704140764935No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGTGTAGTC340.3582341165314508No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGGGTAGTC340.3582341165314508No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGTGTAGTC330.34769781898640817No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCCTGTAGTC330.34769781898640817No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTAGTAGTC320.33716152144136546No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTAGTAGTC320.33716152144136546No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCCTGTAGTC320.33716152144136546No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCTGTAGTC310.32662522389632287No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTAGTAGTC310.32662522389632287No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCCTGTAGTC310.32662522389632287No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTAGTAGTC300.31608892635128016No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTGGTAGTC300.31608892635128016No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTAGTAGTC290.3055526288062375No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCCTGTAGTC280.2950163312611948No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGAGTAGTC280.2950163312611948No Hit
CCTACGGGGGGCAGCAGATGTGGATTAGATACCCTTGTAGTC270.28448003371615216No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGTGTAGTC270.28448003371615216No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCTTGTAGTC270.28448003371615216No Hit
CCTACGGGGGGCTGCAGATGTGGATTAGATACCCTTGTAGTC260.27394373617110945No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGGGTAGTC260.27394373617110945No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGGGTAGTC260.27394373617110945No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGAGTAGTC260.27394373617110945No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCCAGTAGTC260.27394373617110945No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTAGTAGTC250.2634074386260668No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGGGTAGTC250.2634074386260668No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGAGTAGTC240.2528711410810241No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCCTGTAGTC240.2528711410810241No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCCTGTAGTC240.2528711410810241No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGTGTAGTC230.24233484353598148No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCCAGTAGTC230.24233484353598148No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTGGTAGTC230.24233484353598148No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGGGTAGTC230.24233484353598148No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTGGTAGTC220.23179854599093877No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCCAGTAGTC220.23179854599093877No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTAGTAGTC210.22126224844589612No Hit
CCTACGGGCGGCTGCAGATGTGGATTAGATACCCTTGTAGTC210.22126224844589612No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGAGTAGTC210.22126224844589612No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGGGTAGTC210.22126224844589612No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCCGGTAGTC200.21072595090085344No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGTGTAGTC200.21072595090085344No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTGGTAGTC200.21072595090085344No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTGGTAGTC200.21072595090085344No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGTGTAGTC200.21072595090085344No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTTGTAGTC200.21072595090085344No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTGGTAGTC200.21072595090085344No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGAGTAGTC200.21072595090085344No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCTAGTAGTC190.2001896533558108No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTTGTAGTC190.2001896533558108No Hit
CCTACGGGTGGCTGCAGATGTGGATTAGATACCCTTGTAGTC180.1896533558107681No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCGTGTAGTC180.1896533558107681No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCTGTAGTC180.1896533558107681No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCAGTAGTC180.1896533558107681No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCGTGTAGTC170.1791170582657254No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTGGTAGTC170.1791170582657254No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCTTGTAGTC170.1791170582657254No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCTGGTAGTC170.1791170582657254No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCGAGTAGTC170.1791170582657254No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCTTGTAGTC160.16858076072068273No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCCGGTAGTC160.16858076072068273No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGGATTAGATACCCTTGTAGTC160.16858076072068273No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCGTGTAGTC160.16858076072068273No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCTTGTAGTC160.16858076072068273No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCCGGTAGTC160.16858076072068273No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCTAGTAGTC160.16858076072068273No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGGGTAGTC150.15804446317564008No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCCTGTAGTC150.15804446317564008No Hit
CCTACGGGCGGCTGCAGATGTGGATTAGATACCCCTGTAGTC150.15804446317564008No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGGATTAGATACCCTTGTAGTC150.15804446317564008No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCGTGTAGTC150.15804446317564008No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCGGGTAGTC150.15804446317564008No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCCAGTAGTC140.1475081656305974No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCCAGTAGTC140.1475081656305974No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCCGGTAGTC140.1475081656305974No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCTAGTAGTC140.1475081656305974No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC130.13697186808555473No Hit
CCTACGGGTGGCTGCAGATGTGGATTAGATACCCCTGTAGTC130.13697186808555473No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCTTGTAGTC130.13697186808555473No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCGTGTAGTC120.12643557054051205No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCTTGTAGTC120.12643557054051205No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCCAGTAGTC120.12643557054051205No Hit
CCTACGGGGGGCTGCAGATGTGGATTAGATACCCGTGTAGTC120.12643557054051205No Hit
CCTACGGGTGGCTGCAGATGTGGATTAGATACCCGAGTAGTC120.12643557054051205No Hit
CCTACGGGGGGCAGCAGATGTGGATTAGATACCCGTGTAGTC120.12643557054051205No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGGATTAGATACCCCTGTAGTC120.12643557054051205No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGAGTAGTC120.12643557054051205No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCTAGTAGTC110.11589927299546939No Hit
CCTACGGGGGGCAGCAGATGTGGATTAGATACCCCTGTAGTC110.11589927299546939No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCCGGTAGTC110.11589927299546939No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGAGTAGTC110.11589927299546939No Hit
CCTACGGGGGGCTGCAGATGTGGATTAGATACCCGGGTAGTC110.11589927299546939No Hit
CCTACGGGGGGCTGCAGATGTGGATTAGATACCCCTGTAGTC110.11589927299546939No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTAGTAGTC110.11589927299546939No Hit
CCTACGGGTGGCTGCAGATGTGGATTAGATACCCTAGTAGTC110.11589927299546939No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGGATTAGATACCCGTGTAGTC110.11589927299546939No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN110.11589927299546939No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTAGTAGTC110.11589927299546939No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCTAGTAGTC110.11589927299546939No Hit
CCTACGGGCGGCTGCAGATGTGGATTAGATACCCGTGTAGTC110.11589927299546939No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCCAGTAGTC100.10536297545042672No Hit
CCTACGGGGGGCAGCAGATGTGGATTAGATACCCGAGTAGTC100.10536297545042672No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGGATTAGATACCCCTGTAGTC100.10536297545042672No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCCGGTAGTC100.10536297545042672No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCGTGTAGTC100.10536297545042672No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCCTGTAGTC100.10536297545042672No Hit
CCTACGGGGGGCAGCAGATGTGGATTAGATACCCGGGTAGTC100.10536297545042672No Hit
CCTACGGGTGGCTGCAGATGTGGATTAGATACCCGTGTAGTC100.10536297545042672No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCTTGTAGTC100.10536297545042672No Hit
CCTACGGGGGGCAGCAGATGTGGATTAGATACCCTGGTAGTC100.10536297545042672No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGAGTAGTC100.10536297545042672No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCCGGTAGTC100.10536297545042672No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGGGATTAGATACCCGGGTAGTC100.10536297545042672No Hit
CCTACGGGCGGCTGCAGATGTGGATTAGATACCCTAGTAGTC100.10536297545042672No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGGATTAGATACCCGTGTAGTC100.10536297545042672No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCCGGGTAGTC100.10536297545042672No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTGGTAGTC100.10536297545042672No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGGATTAGATACCCCTGTAGTC100.10536297545042672No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)