FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103701
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA48784.703908351896318No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA34163.2940858815247687No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA32693.1523321858034157No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA29102.806144588769636No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA26162.5226371973269304No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA21972.1185909489783126No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA18771.8100114752991774No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA17941.7299736743136518No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA11081.0684564276140056No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA9320.8987377170904812No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6060.5843723782798623No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5880.5670147828854109No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5170.4985487121628528No Hit
GACTACAAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG4850.4676907647949393No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4600.44358299341375684No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4190.40404624834861763No Hit
GACTACAAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT3940.3799384769674352No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3600.3471519078890271No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3290.31725827137636087No Hit
GACTACAGGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG3090.29797205427141493No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3090.29797205427141493No Hit
GACTACACGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG3050.29411481085042573No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3010.2902575674294366No Hit
GACTACTAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG2870.27675721545597437No Hit
GACTACTCGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG2780.2680784177587487No Hit
GACTACAAGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC2770.2671141069035014No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2530.24397064637756627No Hit
GACTACAGGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT2470.2381847812460825No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2430.2343275378250933No Hit
GACTACAAGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT2410.2323989161145987No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2350.2266130509831149No Hit
GACTACACGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT2350.2266130509831149No Hit
GACTACCCGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG2290.22082718585163114No Hit
GACTACTAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT2250.21696994243064197No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2070.1996123470361906No Hit
GACTACAGGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC2060.1986480361809433No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1980.19093354933896492No Hit
GANTACAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1920.18514768420748112No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1920.18514768420748112No Hit
GACTACTAGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1910.18418337335223384No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1890.1822547516417392No Hit
GACTACTCGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1880.18129044078649192No Hit
GACTACACGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1860.17936181907599735No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1830.17646888651025544No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1800.17357595394451356No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1750.16875439966827707No Hit
GACTACACGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1740.1677900888130298No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1730.16682577795778247No Hit
GACTANAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1730.16682577795778247No Hit
GACTACCAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1730.16682577795778247No Hit
GANTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1670.16103991282629868No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1660.1600756019710514No Hit
GACTACCCGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1650.1591112911158041No Hit
GACTACTAGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1630.15718266940530948No Hit
GACTACTGGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1620.1562183585500622No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1610.1552540476948149No Hit
GANTANAAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1570.1513968042738257No Hit
GACTACTCGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1560.15043249341857842No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1540.14850387170808382No Hit
GACTACAGGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1530.14753956085283654No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1510.1456109391423419No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1470.1417536957213527No Hit
GACTACAAGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1450.13982507401085814No Hit
GACTACAAGGGTATCTAATCCATTATTTGAAGCGGGCTCGGAGGAAACGT1440.13886076315561086No Hit
GACTACCAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1410.13596783058986894No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1390.13403920887937434No Hit
GACTACAAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1320.1272890328926433No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1310.12632472203739598No Hit
GACTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1290.12439610032690138No Hit
GACTACCCGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1280.12343178947165409No Hit
GANTACAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1250.12053885690591218No Hit
GACTACTGGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1190.1147529917744284No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1180.11378868091918111No Hit
GACTACAAGGGTATCTAATCCATTTTGAGTTGATTTAGAATATGCAAAGG1170.11282437006393381No Hit
GACTACTCGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1160.11186005920868652No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1160.11186005920868652No Hit
GACTACACGGGTATCTAATCCATTATTTGAAGCGGGCTCGGAGGAAACGT1120.10800281578769733No Hit
GANTACTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1120.10800281578769733No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1100.10607419407720274No Hit
GACTACAGGGGTATCTAATCCATTTTGAGTTGATTTAGAATATGCAAAGG1090.10510988322195543No Hit
GACTACTAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1090.10510988322195543No Hit
GANTANAGGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1050.10125263980096624No Hit
GACTACAGGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1050.10125263980096624No Hit
GANTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1050.10125263980096624No Hit
GANTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1040.10028832894571893No Hit
GANTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1040.10028832894571893No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)