FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91269
Sequences flagged as poor quality0
Sequence length301
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC51065.594451566249219No Hit
CCTACGGGGGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC48835.350118879356628No Hit
CCTACGGGGGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC44394.863644830117565No Hit
CCTACGGGCGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC40024.384840416789928No Hit
CCTACGGGAGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC34003.7252517284072355No Hit
CCTACGGGAGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC24002.629589455346284No Hit
CCTACGGGTGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC13691.4999616518204428No Hit
CCTACGGGCGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC10721.1745499567213402No Hit
CCTANGGGGGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC8940.9795220721164908No Hit
CCTANGGGTGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC8780.9619914757475155No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8050.8820081298140662No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC7750.8491382616222376No Hit
CCTANGGGGGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC7620.8348946520724453No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7520.8239380293418357No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7150.7833985252385804No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC7100.7779202138732757No Hit
CCTANGGGCGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC7050.772441902507971No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA6740.7384763720430815No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC6580.7209457756741062No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG6410.7023195170320701No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG6000.657397363836571No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5580.6113795483680111No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5520.6048055747296454No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA5410.5927532897259749No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC5400.5916576274529138No Hit
CCTANGGGAGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC5370.5883706406337311No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG5160.5653617328994511No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA5100.5587877592610854No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4910.5379701760729273No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC4720.5171525928847692No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG4640.5083872947002817No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4580.5018133210619159No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4550.49852633424273307No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4430.4853783869660016No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA4380.4799000756006968No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA4250.46565646605090444No Hit
CCTANGGGAGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC4220.46236947923172167No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA4160.4557955055933559No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG4050.4437432205896854No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3660.4010123919403083No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3630.3977254051211254No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA3550.38896010693663785No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA3470.3801948087521502No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC3290.3604728878370531No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA3150.3451336160141998No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2850.31226374782237126No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2670.2925418269072741No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA2640.28925484008809127No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA2610.2859678532689084No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2600.28487219099584743No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2510.2750112305382989No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2420.2651502700807503No Hit
CCTANGGGTGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC2390.26186328326156744No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2290.25090666053095795No Hit
CCTACGGGTGGCTGCAGCTTTGAGGAAGATCAGCAGTCAGCTTCAGGTAG2240.24542834916565318No Hit
CCTACGGGGGGCAGCAGCTTTGAGGAAGATCAGCAGTCAGCTTCAGGTAG2220.24323702461953128No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA2140.23447172643504366No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2030.22241944143137318No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2010.22022811688525132No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1980.21694113006606844No Hit
CCTACGGGGGGCTGCAGCTTTGAGGAAGATCAGCAGTCAGCTTCAGGTAG1970.21584546779300748No Hit
CCTANGGGCGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC1860.203793182789337No Hit
CCTACGGGCGGCTGCAGCTTTGAGGAAGATCAGCAGTCAGCTTCAGGTAG1850.20269752051627607No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1790.19612354687791037No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT1760.1928365600587275No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1760.1928365600587275No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1750.19174089778566655No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1750.19174089778566655No Hit
CCTACGGGGGGCAGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC1740.1906452355126056No Hit
CCTACGGGTGGCTGCAGAAGGTTGCAGTAGGCCTTCCACTCAGAGGCACC1730.18954957323954463No Hit
CCTACGGGTGGCTGCAGTACCAAGGAGATGTGTGAAGAGGAGTCATCAAG1720.18845391096648367No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1710.18735824869342274No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1680.1840712618742399No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC1630.17859295050893514No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1620.17749728823587418No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1600.17530596368975226No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1590.1742103014166913No Hit
CCTACGGGGGGCAGCAGAAGGTTGCAGTAGGCCTTCCACTCAGAGGCACC1580.17311463914363037No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC1550.16982765232444752No Hit
CCTACGGGTGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC1540.16873199005138656No Hit
CCTACGGGAGGCAGCAGCTTTGAGGAAGATCAGCAGTCAGCTTCAGGTAG1540.16873199005138656No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1490.1632536786860818No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1480.16215801641302083No Hit
CCTACGGGGGGCTGCAGTACCAAGGAGATGTGTGAAGAGGAGTCATCAAG1450.158871029593838No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1450.158871029593838No Hit
CCTACGGGCGGCTGCAGTACCAAGGAGATGTGTGAAGAGGAGTCATCAAG1440.15777536732077704No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1420.15558404277465515No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1410.1544883805015942No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1390.1522970559554723No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC1390.1522970559554723No Hit
CCTACGGGGGGCAGCAGTACCAAGGAGATGTGTGAAGAGGAGTCATCAAG1370.15010573140935038No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1360.14901006913628942No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1350.14791440686322846No Hit
CCTACGGGTGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC1350.14791440686322846No Hit
CCTANGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1340.14681874459016753No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1310.14353175777098467No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT1300.14243609549792371No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC1290.14134043322486278No Hit
CCTANGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1290.14134043322486278No Hit
CCTACGGGCGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC1270.13914910867874086No Hit
CCTACGGGGGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC1270.13914910867874086No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1260.1380534464056799No Hit
CCTACGGGGGGCTGCAGAAGGTTGCAGTAGGCCTTCCACTCAGAGGCACC1260.1380534464056799No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1250.13695778413261894No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1250.13695778413261894No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1230.13476645958649705No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC1220.1336707973134361No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1200.1314794727673142No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1200.1314794727673142No Hit
CCTANGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1170.12819248594813135No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1170.12819248594813135No Hit
CCTACGGGAGGCAGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC1160.12709682367507041No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1150.12600116140200945No Hit
CCTACGGGAGGCTGCAGCTTTGAGGAAGATCAGCAGTCAGCTTCAGGTAG1140.12490549912894848No Hit
CCTACGGGCGGCTGCAGAAGGTTGCAGTAGGCCTTCCACTCAGAGGCACC1140.12490549912894848No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1130.12380983685588755No Hit
CCTACGGGGGGCAGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC1110.12161851230976564No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1050.11504453867139994No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1030.11285321412527803No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1020.11175755185221707No Hit
CCTACGGGAGGCAGCAGTACCAAGGAGATGTGTGAAGAGGAGTCATCAAG1020.11175755185221707No Hit
CCTANGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1020.11175755185221707No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1010.11066188957915611No Hit
CCTANGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1010.11066188957915611No Hit
CCTANGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC990.10847056503303422No Hit
CCTACGGGAGGCAGCAGAAGGTTGCAGTAGGCCTTCCACTCAGAGGCACC970.10627924048691231No Hit
CCTANGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC960.10518357821385135No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC960.10518357821385135No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG960.10518357821385135No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG950.10408791594079042No Hit
CCTANGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA930.1018965913946685No Hit
CCTACGGGGGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC930.1018965913946685No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)