FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91269
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT57536.303345056919656No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT38834.254456606295676No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT38284.194195181277323No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT33903.7142951056766265No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT29193.198238175064918No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT25422.785173498120939No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT20442.239533686136585No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT19502.1365414324688556No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT11551.2654899253853993No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA8920.9773307475703689No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7440.815172731157348No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6700.7340937229508376No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6070.6650669997479977No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG5990.6563017015635101No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5900.6464407411059615No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5380.589466302906792No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5170.566457395172512No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4990.5467354742574149No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4900.5368745137998663No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4730.5182482551578301No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4700.5149612683386473No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4610.5051003078810987No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG4510.49414368515048923No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG4320.47332610196233116No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG4260.4667521283239654No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4010.4393605714974417No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3820.4185429883092836No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3620.39662974284806446No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG3570.39115143148275977No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3570.39115143148275977No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3540.3878644446635769No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3490.38238613329827215No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3470.3801948087521502No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3430.3758121596599064No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3320.363759874656236No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG3190.34951626510644357No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3140.3440379537411388No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG3050.3341769932835903No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3030.3319856687374684No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2980.3265073573721636No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2830.31007242327624934No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG2810.3078810987301274No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG2810.3078810987301274No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2710.2969244759995179No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2660.29144616463421313No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG2640.28925484008809127No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG2600.28487219099584743No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2490.272819905992177No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2270.24871533598483606No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG2230.24433268689259222No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2210.24214136234647032No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2120.23228040188892174No Hit
GANTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2110.23118473961586083No Hit
GACTACAAGGGTATCTAATCCCTTCCTTTTGCAGAGGACACATCAGGGAA2050.2246107659774951No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2020.22132377915831222No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG2000.21913245461219036No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG2000.21913245461219036No Hit
GANTANAAGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1980.21694113006606844No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1970.21584546779300748No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1940.21255848097382463No Hit
GACTACAAGGGTATCTAATCCAAAATATATAGGTTGTCAACTGGGTCACG1920.2103671564277027No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTGAG1910.2092714941546418No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG1900.20817583188158084No Hit
GACTACAAGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT1890.20708016960851988No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1800.19721920915097133No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1740.1906452355126056No Hit
GANTACAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1720.18845391096648367No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1710.18735824869342274No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1700.18626258642036178No Hit
GACTANAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1700.18626258642036178No Hit
GACTACTAGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT1660.181879937328118No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1640.17968861278199608No Hit
GACTACAAGGGTATCTAATCCCCAGGCATCCATGCAGACACCATGTTCTG1630.17859295050893514No Hit
GACTACAGGGGTATCTAATCCCTTCCTTTTGCAGAGGACACATCAGGGAA1590.1742103014166913No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG1590.1742103014166913No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1520.16654066550526467No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1510.1654450032322037No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1500.16434934095914275No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1480.16215801641302083No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1470.1610623541399599No Hit
GANTACACGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1460.15996669186689894No Hit
GACTACACGGGTATCTAATCCCTTCCTTTTGCAGAGGACACATCAGGGAA1450.158871029593838No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG1440.15777536732077704No Hit
GACTACTAGGGTATCTAATCCCTTCCTTTTGCAGAGGACACATCAGGGAA1440.15777536732077704No Hit
GACTACAGGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT1400.15339271822853323No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1390.1522970559554723No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1370.15010573140935038No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1360.14901006913628942No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1340.14681874459016753No Hit
GACTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1330.14572308231710657No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCCCAG1320.14462742004404563No Hit
GANTANACGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1310.14353175777098467No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1300.14243609549792371No Hit
GANTACAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1300.14243609549792371No Hit
GANTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1270.13914910867874086No Hit
GACTACAGGGGTATCTAATCCCCAGGCATCCATGCAGACACCATGTTCTG1240.135862121859558No Hit
GANTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1230.13476645958649705No Hit
GACTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1230.13476645958649705No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1220.1336707973134361No Hit
GACTACACGGGTATCTAATCCAAAATATATAGGTTGTCAACTGGGTCACG1200.1314794727673142No Hit
GANTANAGGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1200.1314794727673142No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTGAG1190.13038381049425327No Hit
GACTANAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1190.13038381049425327No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTGAG1190.13038381049425327No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1160.12709682367507041No Hit
GACTACACGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT1160.12709682367507041No Hit
GACTACTAGGGTATCTAATCCAAAATATATAGGTTGTCAACTGGGTCACG1160.12709682367507041No Hit
GANTANTAGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1160.12709682367507041No Hit
GANTACTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1150.12600116140200945No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTGAG1130.12380983685588755No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1120.12271417458282659No Hit
GACTACTCGGGTATCTAATCCCTTCCTTTTGCAGAGGACACATCAGGGAA1120.12271417458282659No Hit
GANTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1110.12161851230976564No Hit
GANTANTCGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1070.11723586321752183No Hit
GACTACAGGGGTATCTAATCCAAAATATATAGGTTGTCAACTGGGTCACG1070.11723586321752183No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1060.11614020094446087No Hit
GACTACAAGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG1060.11614020094446087No Hit
GACTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1040.11394887639833898No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCCCAG1010.11066188957915611No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCCCAG1000.10956622730609518No Hit
GANTACTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1000.10956622730609518No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1000.10956622730609518No Hit
GACTANTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1000.10956622730609518No Hit
GACTANACGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT980.10737490275997327No Hit
GACTACTCGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT960.10518357821385135No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG950.10408791594079042No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG950.10408791594079042No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTGAG940.10299225366772946No Hit
GACTACCCGGGTATCTAATCCCTTCCTTTTGCAGAGGACACATCAGGGAA940.10299225366772946No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCCCAG940.10299225366772946No Hit
GACTANTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT940.10299225366772946No Hit
GANTANTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT940.10299225366772946No Hit
GACTACTCGGGTATCTAATCCAAAATATATAGGTTGTCAACTGGGTCACG920.10080092912160755No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG920.10080092912160755No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)