FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104728
Sequences flagged as poor quality0
Sequence length301
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG22542.152241998319456No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG22342.1331449087159116No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG19341.8466885646627456No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT19301.8428691467420366No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG17071.6299365976625162No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT16721.5965166908563133No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT16581.5831487281338323No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG15061.4380108471468949No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCG14931.425597738904591No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT14581.3921778320983882No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCG14351.3702161790543121No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCG12961.2374914063096785No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11801.1267282866091208No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCG11281.0770758536399052No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG10931.0436559468337026No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCG10310.9844549690627149No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG9880.9433962264150944No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG9460.9032923382476511No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8450.8068520357497517No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGGCAATGGAGGAAACTCTGAC8400.8020777633488657No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC8330.7953937819876251No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGGCAATGGAGGAAACTCTGAC8020.7657932931021312No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG7990.7629287296615996No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC7830.7476510579787641No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG7620.7275991138950424No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT7600.7256894049346879No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC7480.7142311511725613No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGGAAGCCTGAC7390.7056374608509663No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG7140.6817660988465358No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGGCAATGGAGGAAACTCTGAC6970.665533572683523No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC6910.6598044458024598No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGGAAGCCTGAC6760.6454816285998014No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6610.6311588113971431No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6480.6187457031548392No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCG6480.6187457031548392No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC6460.6168359941944848No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6340.6053777404323581No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6250.5967840501107632No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC6230.5948743411504087No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGGAAGCCTGAC6060.578641814987396No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGGCAATGGAGGAAACTCTGAC5930.566228706745092No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG5860.5595447253838515No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5670.5414024902604844No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC5580.5328087999388893No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5310.5070277289741043No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5260.5022534565732183No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5250.501298602093041No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5210.4974791841723321No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5190.4955694752119777No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGGAAGCCTGAC5100.48697578489038273No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGGCAATGGAGGAAACTCTGAC5000.4774272400886105No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4800.4583301504850661No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG4630.4420976243220533No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGGAAGCCTGAC4510.4306393705599267No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4500.4296845160797495No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4460.42586509815904056No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG4380.4182262623176228No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC4230.4039034451149645No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4190.40008402719425556No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4060.3876709189519517No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4010.3828966465510656No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3960.3781223741501795No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3910.3733481017492934No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3780.36093499350698954No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTATCCG3780.36093499350698954No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGGCAATGGAGGAAACTCTGAC3690.3523413031853946No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCG3590.34279275838362233No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3510.33515392254220455No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3460.3303796501413185No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC3390.3236956687800779No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3370.32178595981972347No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTATCCG3330.3179665418990146No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGGAAGCCTGAC3270.31223741501795127No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3210.30650828813688796No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCG3170.3026888702161791No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTATCCG3100.2960048888549385No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2970.2835917806126347No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2950.2816820716522802No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2880.27499809029103967No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2850.27213352685050796No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2850.27213352685050796No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTATCCG2740.2616301275685586No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTATCCG2720.2597204186082041No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG2710.25876556412802687No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2660.2539912917271408No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2560.24444274692536858No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCG2380.22725536628217857No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2320.22152623940111527No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCG2310.22057138492093806No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG2170.20720342219845694No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC2070.19765487739668475No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTATCCG2030.19383545947597586No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2000.1909708960354442No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1960.18715147811473531No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGGCAATGGAGGAAACTCTGAC1950.1861966236345581No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1880.17951264227331754No Hit
CCTANGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1860.1776029333129631No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCG1820.1737835153922542No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG1800.17187380643189978No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1790.17091895195172255No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG1670.1594606981895959No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCG1640.15659613474906425No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1630.15564128026888702No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCG1630.15564128026888702No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGGAAGCCTGAC1620.1546864257887098No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG1580.15086700786800092No Hit
CCTANGGGTGGCTGCAGTGAGGAATATTGGGCAATGGAGGAAACTCTGAC1550.14800244442746924No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1550.14800244442746924No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1550.14800244442746924No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1510.14418302650676038No Hit
CCTANGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1500.14322817202658314No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGGCAATGGAGGAAACTCTGAC1500.14322817202658314No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1470.14036360858605149No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1470.14036360858605149No Hit
CCTANGGGGGGCTGCAGTGAGGAATATTGGGCAATGGAGGAAACTCTGAC1420.1355893361851654No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1390.13272477274463373No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG1370.1308150637842793No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGCGGAAGCCTGAC1330.1269956458635704No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1320.12604079138339316No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1310.12508593690321596No Hit
CCTANGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1310.12508593690321596No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1300.12413108242303873No Hit
CCTACGGGTGGCTGCAGTAATACGGAGGGTGCAAGCGTTAATCGGAATTA1290.1231762279428615No Hit
CCTANGGGAGGCTGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCG1280.12222137346268429No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1280.12222137346268429No Hit
CCTANGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1280.12222137346268429No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTATCCG1250.11935681002215262No Hit
CCTANGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGGAAGCCTGAC1220.11649224658162097No Hit
CCTACGGGGGGCAGCAGTAATACGGAGGGTGCAAGCGTTAATCGGAATTA1220.11649224658162097No Hit
CCTANGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1210.11553739210144373No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1190.11362768314108929No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG1130.10789855626002598No Hit
CCTANGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGGAAGCCTGAC1120.10694370177984874No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1110.10598884729967153No Hit
CCTANGGGGGGCAGCAGTGAGGAATATTGGGCAATGGAGGAAACTCTGAC1110.10598884729967153No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1110.10598884729967153No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1100.10503399281949431No Hit
CCTANGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1080.10312428385913987No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1070.10216942937896265No Hit
CCTANGGGTGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1070.10216942937896265No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1050.10025972041860821No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1050.10025972041860821No Hit
CCTACGGGTGGCTGCAGTGGGGAATGTTGCACAATGGGGGAAACCCTGAT1050.10025972041860821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)