FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138974
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC96766.962453408551241No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC87656.306935110164491No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC81675.876638795745967No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC73785.308906701973031No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC64724.65698619885734No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC47973.451724783052945No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC24951.79529984025789No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC19811.4254464863931382No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC16831.2110178882380878No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC14941.0750212269920991No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC13320.9584526602098233No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC12910.9289507389871486No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC11190.8051865816627571No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC9510.6843006605552118No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8330.5993926921582454No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8260.5943557787787643No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7900.5684516528271475No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7490.5389497316044728No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6750.48570236159281593No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5970.4295767553643128No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4800.345388346021558No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4790.34466878696734643No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4690.3374731964252306No Hit
CCTANGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4460.3209233381783643No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4280.30797127520255585No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4090.29429965317253587No Hit
CCTANGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3440.24752831464878322No Hit
CCTACGGGTGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3020.21730683437189688No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2770.19931785801660745No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2750.19787873990818428No Hit
CCTACGGGGGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2680.19284182652870321No Hit
CCTACGGGCGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2560.18420711787816424No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2490.1791702044986832No Hit
CCTACGGGGGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2440.17557240922762532No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2310.16621814152287478No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2290.1647790234144516No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2280.16405946436024005No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2260.16262034625181687No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2230.16046166908918216No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2220.15974211003497057No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2160.1554247557097011No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2140.15398563760127792No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2010.14463136989652742No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1950.14031401557125794No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1930.1388748974628348No Hit
CCTACGGGAGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1920.1381553384086232No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1890.13599666124598847No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1810.13024018881229582No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1790.12880107070387267No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1740.12520327543281476No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1730.1244837163786032No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1700.12232503921596845No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1650.11872724394491056No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1580.11369033056542951No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1560.11225121245700634No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1550.11153165340279476No Hit
CCTACGGGTGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1550.11153165340279476No Hit
CCTANGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1530.11009253529437162No Hit
CCTANGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1530.11009253529437162No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1520.10937297624016003No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1500.10793385813173688No Hit
CCTACGGGGGGCAGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1490.10721429907752529No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1490.10721429907752529No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT1470.10577518096910213No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1440.10361650380646739No Hit
CCTACGGGTGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1420.10217738569804424No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1410.10145782664383265No Hit
CCTANGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1400.10073826758962108No Hit
CCTANGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1390.10001870853540949No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1390.10001870853540949No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)