FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89858
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC47735.311714037703933No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC44024.898840392619467No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC40814.541610095929133No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC36974.114269180262191No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC31053.4554519352756574No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC23192.580738498519887No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC12641.4066638474036814No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT10991.2230407977030426No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC9571.065013688263705No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT9091.0115960738053373No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8920.9926773353513321No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT8540.950388390571791No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8120.9036479779207194No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7540.8391016937835252No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7320.8146186204901066No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7060.7856840793251574No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6700.7456208684813818No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6560.7300407309310245No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6460.718912061252198No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6260.6966547218945447No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6220.6922032540230141No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5300.5898194929778094No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4920.5475305481982684No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4920.5475305481982684No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4220.46962986044648225No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3400.37837476908010415No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2730.303812682231966No Hit
CCTANGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2460.2737652740991342No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2200.24483073293418506No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2010.22368626054441454No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1890.2103318569298226No Hit
CCTANGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1860.20699325602617463No Hit
CCTACGGGTGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1840.20476752209040933No Hit
CCTACGGGTGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1830.20365465512252665No Hit
CCTACGGGGGGCAGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1770.1969774533152307No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1730.19252598544370006No Hit
CCTACGGGGGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1730.19252598544370006No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1710.19030025150793475No Hit
CCTACGGGCGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1660.18473591666852146No Hit
CCTACGGGGGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1540.17138151305392954No Hit
CCTACGGGGGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1510.16804291215028155No Hit
CCTACGGGCGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1430.15913997640722027No Hit
CCTANGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1400.1558013755035723No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1390.15468850853568966No Hit
CCTACGGGTGGCTGCAGGGAGCTATGATCGCACTACTGCACTCCAGCCTG1380.15357564156780698No Hit
CCTACGGGTGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1370.15246277459992433No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1360.15134990763204167No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1340.14912417369627634No Hit
CCTACGGGAGGCAGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1260.14022123795321506No Hit
CCTACGGGGGGCTGCAGGGAGCTATGATCGCACTACTGCACTCCAGCCTG1240.13799550401744975No Hit
CCTACGGGGGGCAGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1220.13576977008168445No Hit
CCTACGGGGGGCAGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA1220.13576977008168445No Hit
CCTACGGGAGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1210.13465690311380177No Hit
CCTACGGGTGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1200.13354403614591911No Hit
CCTANGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1200.13354403614591911No Hit
CCTACGGGGGGCAGCAGGGAGCTATGATCGCACTACTGCACTCCAGCCTG1180.13131830221015378No Hit
CCTACGGGTGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA1170.13020543524227116No Hit
CCTACGGGGGGCAGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1170.13020543524227116No Hit
CCTACGGGTGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1140.12686683433862317No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1130.1257539673707405No Hit
CCTANGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1110.12352823343497518No Hit
CCTACGGGGGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA1110.12352823343497518No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1100.12241536646709253No Hit
CCTACGGGGGGCAGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1080.1201896325313272No Hit
CCTACGGGCGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1050.11685103162767924No Hit
CCTACGGGCGGCTGCAGGGAGCTATGATCGCACTACTGCACTCCAGCCTG1040.11573816465979658No Hit
CCTANGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1020.11351243072403125No Hit
CCTACGGGAGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1010.11239956375614858No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1010.11239956375614858No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT990.11017382982038326No Hit
CCTACGGGGGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC980.10906096285250061No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT980.10906096285250061No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC980.10906096285250061No Hit
CCTACGGGAGGCAGCAGGGAGCTATGATCGCACTACTGCACTCCAGCCTG970.10794809588461796No Hit
CCTACGGGAGGCAGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC960.10683522891673529No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC940.10460949498096997No Hit
CCTACGGGCGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA940.10460949498096997No Hit
CCTACGGGGGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA940.10460949498096997No Hit
CCTACGGGCGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG910.101270894077322No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC910.101270894077322No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)