FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115303
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA71546.204521998560315No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA48514.207175875736104No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA47034.078818417560688No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA43543.7761376547010923No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA38523.340763033052045No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA31792.7570835104030254No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA25622.221971674631189No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA25022.169934867262777No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA15791.3694353139120403No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA7210.62530896854375No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA5880.5099607122104368No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4790.41542717882448854No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4670.40501981735080617No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG4010.347779329245553No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3950.3425756485087118No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3830.33216828703502943No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3780.3278318864209951No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3760.3260973261753814No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3190.27666235917539006No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3050.26452043745609394No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3000.26018403684205965No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2770.24023659401750172No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2680.2324310729122399No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2610.22636011205259188No Hit
GANTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2600.225492831929785No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2570.2228909915613644No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2490.21595275057890947No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2440.21161634996487516No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2410.20901450959645457No Hit
GANTANAAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2260.19600530775435157No Hit
GANTACAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2240.19427074750873785No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2210.19166890714031726No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2110.1829961059122486No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2110.1829961059122486No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2060.1786597052982143No Hit
GACTACAAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1970.17085418419295248No Hit
GACTACAAGGGTATCTAATCCATTCTGAGTTGGTTTTTGTACATGGTGTA1960.16998690407014563No Hit
GANTACAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1820.15784498235084948No Hit
GACTANAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1810.15697770222804264No Hit
GACTACAAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1690.14657034075436023No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1690.14657034075436023No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1670.14483578050874651No Hit
GANTACACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1670.14483578050874651No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1660.14396850038593964No Hit
GACTANACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1660.14396850038593964No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1600.13876481964909848No Hit
GACTACAAGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1580.13703025940348473No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1570.13616297928067786No Hit
GACTACAGGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1550.13442841903506414No Hit
GACTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1520.13182657866664355No Hit
GANTANACGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1470.1274901780526092No Hit
GANTANAGGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1450.12575561780699548No Hit
GACTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1450.12575561780699548No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1450.12575561780699548No Hit
GANTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1440.12488833768418861No Hit
GANTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1420.12315377743857489No Hit
GACTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1400.12141921719296116No Hit
GANTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1370.11881737682454055No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1370.11881737682454055No Hit
GACTANAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1360.11795009670173369No Hit
GACTANTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1350.11708281657892683No Hit
GACTANTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1340.11621553645611996No Hit
GACTACAGGGGTATCTAATCCATTCTGAGTTGGTTTTTGTACATGGTGTA1340.11621553645611996No Hit
GACTACAAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1330.1153482563333131No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1320.11448097621050624No Hit
GANTACTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1310.11361369608769936No Hit
GANTACTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1310.11361369608769936No Hit
GACTACAAGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1290.11187913584208564No Hit
GANTANCCGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1280.11101185571927878No Hit
GACTACAGGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1280.11101185571927878No Hit
GACTACACGGGTATCTAATCCATTCTGAGTTGGTTTTTGTACATGGTGTA1280.11101185571927878No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1270.11014457559647192No Hit
GACTACTAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1270.11014457559647192No Hit
GACTACAAGGGTATCTAATCCATTATTTGAAGCGGGCTCGGAGGAAACGT1270.11014457559647192No Hit
GACTACTAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1260.10927729547366503No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1250.10841001535085816No Hit
GACTACACGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1240.1075427352280513No Hit
GACTACTAGGGTATCTAATCCATTCTGAGTTGGTTTTTGTACATGGTGTA1240.1075427352280513No Hit
GACTANTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1230.10667545510524444No Hit
GANTANTAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1220.10580817498243758No Hit
GACTACAGGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1190.10320633461401699No Hit
GACTACACGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1180.10233905449121013No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)