FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107920
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC38843.598962194217939No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC37033.4312453669384726No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC32573.0179762787249818No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC30942.8669384729429206No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC26682.4722016308376573No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC19911.844885100074129No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC10170.94236471460341No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC9450.875648628613788No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8950.8293180133432172No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8370.7755744996293551No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7920.7338769458858413No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7250.6717939214232765No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6510.6032246108228317No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6310.5846923647146034No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6250.5791326908821349No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5930.5494810971089695No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5300.4911045218680504No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4680.43365455893254257No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG4480.41512231282431433No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG4340.4021497405485545No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4270.3956634544106745No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG4200.3891771682727947No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3940.3650852483320978No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG3830.3548925129725723No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3750.34747961452928094No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3410.31597479614529284No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG3340.3094885100074129No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3320.3076352853965901No Hit
CCTACGGGGGGCAGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG3070.28446997776130467No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG3060.28354336545589326No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2980.2761304670126019No Hit
CCTACGGGTGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG2920.27057079318013344No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG2730.2529651593773165No Hit
CCTACGGGGGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG2630.2436990363232024No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2600.24091919940696813No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2570.2381393624907339No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2570.2381393624907339No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2550.23628613787991104No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2420.22424017790956263No Hit
CCTACGGGAGGCAGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG2390.22146034099332837No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2390.22146034099332837No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2370.21960711638250555No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2360.21868050407709413No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2290.21219421793921422No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2260.20941438102298002No Hit
CCTACGGGCGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG2250.20848776871756858No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2230.20663454410674575No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2220.20570793180133434No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2150.1992216456634544No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2040.18902891030392882No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2030.1881022979985174No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1930.17883617494440326No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1880.17420311341734618No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1880.17420311341734618No Hit
CCTANGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1820.16864343958487768No Hit
CCTACGGGAGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1760.16308376575240918No Hit
CCTACGGGTGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1750.1621571534469978No Hit
CCTANGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1690.15659747961452927No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1680.15567086730911786No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1650.15289103039288363No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1640.1519644180874722No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1610.14918458117123795No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1610.14918458117123795No Hit
CCTACGGGTGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1610.14918458117123795No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1600.14825796886582654No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1580.1464047442550037No Hit
CCTACGGGGGGCAGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1570.14547813194959228No Hit
CCTANGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1550.14362490733876948No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1550.14362490733876948No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1500.1389918458117124No Hit
CCTANGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1490.13806523350630096No Hit
CCTANGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1440.1334321719792439No Hit
CCTACGGGTGGCTGCAGGCAGCAGGCAGCGCGCGGTACTGGCGAGTTCTC1420.13157894736842105No Hit
CCTACGGGGGGCAGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1380.12787249814677537No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1350.12509266123054114No Hit
CCTACGGGGGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1330.12323943661971831No Hit
CCTACGGGCGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1310.12138621200889549No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1310.12138621200889549No Hit
CCTANGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1290.11953298739807265No Hit
CCTACGGGGGGCAGCAGGCAGCAGGCAGCGCGCGGTACTGGCGAGTTCTC1280.11860637509266124No Hit
CCTACGGGGGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1260.1167531504818384No Hit
CCTACGGGGGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1260.1167531504818384No Hit
CCTACGGGTGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1250.11582653817642699No Hit
CCTACGGGAGGCAGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1230.11397331356560415No Hit
CCTANGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1220.11304670126019273No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1210.11212008895478132No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAACCG1200.1111934766493699No Hit
CCTACGGGGGGCTGCAGGCAGCAGGCAGCGCGCGGTACTGGCGAGTTCTC1190.11026686434395848No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1180.10934025203854707No Hit
CCTACGGGTGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1170.10841363973313564No Hit
CCTACGGGCGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1140.10563380281690139No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1100.10192735359525575No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1100.10192735359525575No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1090.10100074128984433No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAACCG1080.10007412898443292No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)