FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170313
Sequences flagged as poor quality0
Sequence length301
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC117806.916676941865858No Hit
CCTACGGGGGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC114746.73700774456442No Hit
CCTACGGGGGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC104896.158660818610441No Hit
CCTACGGGCGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC91185.353672356191248No Hit
CCTACGGGAGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC81294.77297681327908No Hit
CCTACGGGAGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC56213.300393980494736No Hit
CCTACGGGTGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC32031.880655029269639No Hit
CCTACGGGCGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC25481.4960690023662315No Hit
CCTANGGGTGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC20761.2189322012999595No Hit
CCTANGGGGGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC19641.1531709264706747No Hit
CCTANGGGGGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC18291.0739051041318044No Hit
CCTANGGGCGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC16210.9517770223059895No Hit
CCTANGGGAGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC14130.8296489404801747No Hit
CCTANGGGAGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC9180.5390075919043174No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6840.4016134998502756No Hit
CCTACGGGTGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC6510.3822374099452185No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6480.38047594722657696No Hit
CCTACGGGGGGCAGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC6230.3657970912378973No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5960.3499439267701232No Hit
CCTACGGGGGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC5360.31471467239729206No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5350.31412751815774487No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5330.3129532096786505No Hit
CCTANGGGTGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC5310.3117789011995561No Hit
CCTACGGGCGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC5230.3070816672831786No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4600.27009095019170587No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG4510.2648065620357812No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG4500.264219407796234No Hit
CCTACGGGAGGCAGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC4200.24660478060981839No Hit
CCTANGGGCGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC4180.245430472130724No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4140.24308185517253528No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3940.23133877038159154No Hit
CCTACGGGTGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC3690.21665991439291185No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG3590.21078837199744No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG3560.20902690927879844No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3550.20843975503925127No Hit
CCTACGGGGGGCAGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC3460.20315536688332658No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3340.19610951600876034No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG3290.1931737448110244No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3240.19023797361328848No Hit
CCTACGGGAGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC3110.18260496849917507No Hit
CCTACGGGGGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC2970.17438480914551444No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2950.17321050066642008No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGAGAGATCCTGAA2920.1714490379477785No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2900.17027472946868413No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2890.16968757522913694No Hit
CCTACGGGCGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC2780.1632288785941179No Hit
CCTACGGGAGGCAGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC2780.1632288785941179No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGAGAGATCCTGAA2690.15794449043819322No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2670.15677018195909884No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2660.15618302771955164No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2500.14678855988679668No Hit
CCTACGGGTGGCTGCAGCCGCGGTAAGACAGAGGATGCAAGCGTTATCCG2410.14150417173087199No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2410.14150417173087199No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGAGAGATCCTGAA2380.13974270901223043No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT2370.13915555477268324No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2360.13856840053313604No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2300.13504547509585293No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGAGAGATCCTGAA2290.13445832085630574No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2280.13387116661675855No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG2250.132109703898117No Hit
CCTACGGGGGGCAGCAGCCGCGGTAAGACAGAGGATGCAAGCGTTATCCG2220.13034824117947544No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2130.12506385302355075No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT2000.11743084790943734No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1950.11449507671170141No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1930.11332076823260702No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGAGAGATCCTGAA1920.11273361399305984No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1850.10862353431622954No Hit
CCTACGGGGGGCTGCAGCCGCGGTAAGACAGAGGATGCAAGCGTTATCCG1850.10862353431622954No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1820.10686207159758797No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC1790.1051006088789464No Hit
CCTACGGGCGGCTGCAGCCGCGGTAAGACAGAGGATGCAAGCGTTATCCG1780.10451345463939922No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG1770.10392630039985204No Hit
CCTACGGGTGGCTGCAGCATCAGGGAGGAAGCCCCACATGAACTTGAGAA1730.10157768344166329No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)