FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88468
Sequences flagged as poor quality0
Sequence length301
%GC56

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT15051.701180087715332No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT14021.5847538092869737No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13711.5497128905366913No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT13701.5485825383189402No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT13691.5474521861011892No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12481.410679567753312No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11591.3100782203734684No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC11561.3066871637202153No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC11521.302165754849211No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT11061.2501695528326628No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC10791.2196500429533843No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG10101.1416557399285618No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG9901.1190486955735408No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC9331.0546186191617308No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9161.035402631459963No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT9011.0184473481936973No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8961.0127955871049419No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC8790.993579599403174No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT8710.9845367816611655No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC8610.9732332594836551No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC8090.9144549441606004No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT8050.9099335352895963No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG7790.8805443776280688No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC7740.8748926165393137No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG7620.8613283899263011No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT7590.8579373332730478No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG7320.8274178233937696No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT7250.8195053578695122No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7160.8093321879097526No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA6800.7686395080707148No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6770.7652484514174617No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6760.7641180991997106No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG6580.7437717592801917No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6540.7392503504091875No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC6490.7335985893204323No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGCAGGGCTGAA6280.7098611927476602No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG6270.7087308405299091No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA6040.6827327395216349No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6000.6782113306506308No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG5970.6748202739973775No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA5960.6736899217796266No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5800.6556042862956097No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC5610.6341275941583397No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC5570.6296061852873356No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGCAGGGCTGAA5490.620563367545327No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG5250.593434914319302No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA5240.5923045621015508No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGCAGGGCTGAA5230.5911742098837998No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4970.5617850522222725No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG4720.5335262467784962No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGCCGCAGGGCTGAA4660.5267441334719899No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG4440.5018763846814668No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC4250.48039969254419673No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA4220.47700863589094356No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4200.47474793145544153No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4060.4589230004069268No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3860.43631595605190576No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3770.42614278609214634No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGCAGGGCTGAA3640.41144820726138265No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3420.38658045847085953No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3210.36284306189808746No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA3200.36171270968033636No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG3090.3492788352850748No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC3080.34814848306732377No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG2950.33345390423656013No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2830.3198896776235475No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG2820.31875932540579643No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGCAGGGCTGAA2790.3153682687525433No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2660.3006736899217796No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2570.29050051996202014No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG2530.2859791110910159No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2520.28484875887326494No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC2380.2690238278247502No Hit
CCTANGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC2310.26111136230049287No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2310.26111136230049287No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG2310.26111136230049287No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2290.25885065786499073No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG2270.25658995342948865No Hit
CCTANGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC2260.2554596012117376No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2160.24415607903422706No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2140.24189537459872495No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2110.23850431794547178No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2070.2339829090744676No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2070.2339829090744676No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG2040.23059185242121444No Hit
CCTANGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC2020.22833114798571233No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCG1960.22154903467920603No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1950.22041868246145496No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCG1920.21702762580820184No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1860.21024551250169551No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1830.2068544558484424No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCG1810.20459375141294026No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1780.20120269475968713No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA1770.20007234254193607No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1760.19894199032418503No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGCAGGGCTGAA1750.19781163810643396No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1680.1898991725821766No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1670.18876882036442555No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1660.1876384681466745No Hit
CCTANGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC1650.18650811592892347No Hit
CCTANGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC1620.1831170592756703No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1580.1785956504046661No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG1580.1785956504046661No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG1570.17746529818691506No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1570.17746529818691506No Hit
CCTANGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1570.17746529818691506No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1570.17746529818691506No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1520.17181353709815977No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1470.16616177600940452No Hit
CCTANGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1430.16164036713840033No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCG1410.15937966270289822No Hit
CCTANGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1390.1571189582673961No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1390.1571189582673961No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1340.15146719717864088No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCG1310.1480761405253877No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG1310.1480761405253877No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1300.14694578830763666No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA1290.14581543608988562No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1290.14581543608988562No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1280.14468508387213455No Hit
CCTANGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA1260.14242437943663247No Hit
CCTANGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA1230.1390333227833793No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1200.13564226613012614No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1200.13564226613012614No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1190.1345119139123751No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGACAATGGTCGGAAGACTGAT1180.13338156169462406No Hit
CCTANGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA1170.132251209476873No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1160.13112085725912193No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG1140.12886015282361984No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGTCGGAAGACTGAT1110.1254690961703667No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1100.12433874395261564No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGCCGCAGGGCTGAA1090.12320839173486457No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG1060.11981733508161144No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1040.11755663064610933No Hit
CCTANGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGCAGGGCTGAA1040.11755663064610933No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGTCGGAAGACTGAT1030.11642627842835829No Hit
CCTANGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1000.11303522177510512No Hit
CCTANGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC990.11190486955735407No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC980.11077451733960302No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC970.10964416512185197No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGACAATGGTCGGAAGACTGAT970.10964416512185197No Hit
CCTANGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA960.10851381290410092No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT960.10851381290410092No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT950.10738346068634988No Hit
CCTANGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGCAGGGCTGAA910.10286205181534566No Hit
CCTACGGGTGGCTGCAGTGGGGAATCCTGCACAATGGGCGGAAGCCTGAT910.10286205181534566No Hit
CCTANGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGCAGGGCTGAA910.10286205181534566No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT900.10173169959759461No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCG890.10060134737984357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)