FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004833517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12121
Sequences flagged as poor quality0
Sequence length35-251
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3012.483293457635509No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2311.9057833512086464No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2211.8232819074333801No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2031.6747793086379013No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1881.5510271429750022No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1871.5427769985974753No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1661.3695239666694166No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1641.3530236779143634No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1571.2952726672716772No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1471.2127712234964112No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1401.155020212853725No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1401.155020212853725No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1381.1385199240986716No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1291.0642686247009323No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1241.0230179028132993No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1210.9982674696807194No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1200.9900173253031929No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1170.9652668921706129No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1160.9570167477930864No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1160.9570167477930864No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1080.8910155927728736No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1060.8745153040178203No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1000.8250144377526606No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG920.7590132827324478No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG860.7095124164672881No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG840.693012127712235No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG830.6847619833347083No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG830.6847619833347083No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG780.6435112614470754No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG730.6022605395594423No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG710.5857602508043891No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG690.5692599620493358No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG680.5610098176718092No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG650.5362593845392294No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG650.5362593845392294No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG650.5362593845392294No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG650.5362593845392294No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG570.4702582295190166No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG570.4702582295190166No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG570.4702582295190166No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG550.45375794076396336No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG540.4455077963864368No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG530.43725765200891015No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG520.42900750763138357No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG520.42900750763138357No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG500.4125072188763303No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG500.4125072188763303No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG480.3960069301212771No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG460.3795066413662239No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG450.3712564969886973No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG420.3465060638561175No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG410.33825591947859085No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG380.31350548634601105No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG370.3052553419684845No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG370.3052553419684845No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG360.29700519759095784No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG350.28875505321343126No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG350.28875505321343126No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG350.28875505321343126No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG350.28875505321343126No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG350.28875505321343126No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG340.2805049088359046No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG330.272254764458378No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG330.272254764458378No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG330.272254764458378No Hit
GACTACAAGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTCAG310.2557544757033248No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG300.24750433132579822No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG300.24750433132579822No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG300.24750433132579822No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG290.2392541869482716No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAA290.2392541869482716No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAA280.231004042570745No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG280.231004042570745No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG270.2227538981932184No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG270.2227538981932184No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG260.21450375381569178No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG260.21450375381569178No Hit
GACTACACGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTCAG260.21450375381569178No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG250.20625360943816515No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG250.20625360943816515No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG250.20625360943816515No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG250.20625360943816515No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG240.19800346506063854No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG240.19800346506063854No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCAG230.18975332068311196No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG230.18975332068311196No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG230.18975332068311196No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG220.18150317630558535No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG220.18150317630558535No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAA220.18150317630558535No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG220.18150317630558535No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG210.17325303192805874No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG210.17325303192805874No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG210.17325303192805874No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG200.16500288755053213No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAAG200.16500288755053213No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG200.16500288755053213No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG200.16500288755053213No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCTGGCTTTCGCGCCTCAG200.16500288755053213No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG190.15675274317300553No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCAG190.15675274317300553No Hit
GACTACCCGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTCAG190.15675274317300553No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCTGGCTTTCGCGCCTCAG190.15675274317300553No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA190.15675274317300553No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG190.15675274317300553No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG190.15675274317300553No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG190.15675274317300553No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAA190.15675274317300553No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGG180.14850259879547892No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG180.14850259879547892No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAA180.14850259879547892No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG180.14850259879547892No Hit
GACTACAAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCCCCCGTAGG170.1402524544179523No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG170.1402524544179523No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG170.1402524544179523No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCTGGCTTTCGCGCCTCAG170.1402524544179523No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG170.1402524544179523No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG170.1402524544179523No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG170.1402524544179523No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG160.1320023100404257No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA160.1320023100404257No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG160.1320023100404257No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG160.1320023100404257No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG160.1320023100404257No Hit
GACTACTCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCATGAG160.1320023100404257No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAA160.1320023100404257No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG150.12375216566289911No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG150.12375216566289911No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAAG150.12375216566289911No Hit
GACTACACGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCATGAG150.12375216566289911No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG150.12375216566289911No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG150.12375216566289911No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG150.12375216566289911No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG150.12375216566289911No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCATGAG150.12375216566289911No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA140.1155020212853725No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA140.1155020212853725No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCAG140.1155020212853725No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG140.1155020212853725No Hit
GACTACTGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTCAG140.1155020212853725No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCAG140.1155020212853725No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA140.1155020212853725No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG140.1155020212853725No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG140.1155020212853725No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAAG140.1155020212853725No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG140.1155020212853725No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG140.1155020212853725No Hit
GACTACAGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTCAG140.1155020212853725No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAA140.1155020212853725No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG140.1155020212853725No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG130.10725187690784589No Hit
GACTACACGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGG130.10725187690784589No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG130.10725187690784589No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG130.10725187690784589No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG130.10725187690784589No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTTAG130.10725187690784589No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG130.10725187690784589No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG130.10725187690784589No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA130.10725187690784589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)