FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96385
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA50525.2414794833220935No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA34803.6105203091767386No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA33233.4476318929293974No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA30653.1799553872490534No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA26462.745240441977486No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA22052.287700368314572No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA19041.9754111116875033No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA17201.7845100378689631No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA10941.135031384551538No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6910.7169165326554962No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4440.46065259117082535No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4240.4399024744514188No Hit
GACTACAAGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC3870.40151475852051666No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3590.3724645951133475No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3470.36001452508170356No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3330.345489443378119No Hit
GACTACAAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG3020.31332676246303887No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2970.30813923328318726No Hit
GACTACAAGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC2860.2967266690875136No Hit
GACTACAGGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC2480.2573014473206412No Hit
GACTACTAGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC2370.24588888312496757No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2370.24588888312496757No Hit
GACTACAGGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG2290.23758883643720496No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2250.23343881309332368No Hit
GACTACACGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC2210.22928878974944233No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2140.22202624889765005No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2140.22202624889765005No Hit
GACTACAGGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC2130.22098874306167973No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2100.21787622555376873No Hit
GACTACTAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG2020.2095761788660061No Hit
GACTACACGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1970.2043886496861545No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1950.20231363801421381No Hit
GACTACTCGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1940.2012761321782435No Hit
GACTACACGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1830.1898635679825699No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG1820.18882606214659958No Hit
GANTANAAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1790.1857135446386886No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1760.1826010271307776No Hit
GANTACAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1720.1784510037868963No Hit
GACTACCCGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1680.17430098044301498No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1680.17430098044301498No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1660.17222596877107435No Hit
GACTACTCGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1620.16807594542719304No Hit
GANTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1590.16496342791928204No Hit
GACTANAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1570.1628884162473414No Hit
GACTACAAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1570.1628884162473414No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1560.16185091041137106No Hit
GACTACAAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1530.1587383929034601No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1530.1587383929034601No Hit
GACTACTCGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1520.15770088706748975No Hit
GACTACAAGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1490.15458836955957878No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1460.15147585205166778No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1460.15147585205166778No Hit
GACTACTAGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1450.15043834621569746No Hit
GACTACCAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1430.1483633345437568No Hit
GANTACAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1430.1483633345437568No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1420.1473258287077865No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1410.14628832287181615No Hit
GACTACTGGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1380.14317580536390517No Hit
GACTACCCGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1370.14213829952793483No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT1310.13591326451211286No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT1290.13383825284017223No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG1290.13383825284017223No Hit
GACTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1290.13383825284017223No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1280.13280074700420189No Hit
GANTACACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1250.1296882294962909No Hit
GACTACTAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1230.12761321782435026No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1210.12553820615240963No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG1180.12242568864449861No Hit
GACTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1170.12138818280852831No Hit
GACTACAAGGGTATCTAATCCGGGGGAGACAGAGCCGGATGAGAGCTTGG1170.12138818280852831No Hit
GACTACTGGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1170.12138818280852831No Hit
GACTACAGGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1170.12138818280852831No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1160.12035067697255797No Hit
GACTACACGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1160.12035067697255797No Hit
GACTACCAGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1160.12035067697255797No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG1150.11931317113658764No Hit
GACTACAGGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1140.11827566530061731No Hit
GACTACCCGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1130.117238159464647No Hit
GANTACTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1130.117238159464647No Hit
GANTANACGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1120.11620065362867667No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT1120.11620065362867667No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT1110.11516314779270634No Hit
GACTACAAGGGTATCTAATCCATCCCAGTATGGAGCAGATATCCCTTGTA1100.114125641956736No Hit
GANTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1100.114125641956736No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1070.11101312444882502No Hit
GACTANACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1050.10893811277688437No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1050.10893811277688437No Hit
GACTACCGGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1040.10790060694091405No Hit
GANTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1040.10790060694091405No Hit
GANTANTAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1020.1058255952689734No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG1010.10478808943300305No Hit
GANTANAGGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1010.10478808943300305No Hit
GACTANAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1000.10375058359703272No Hit
GACTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1000.10375058359703272No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG980.10167557192509208No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG980.10167557192509208No Hit
GACTACACGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA980.10167557192509208No Hit
GACTACACGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT970.10063806608912175No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA970.10063806608912175No Hit
GACTACCAGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC970.10063806608912175No Hit
GACTACTGGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC970.10063806608912175No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)