FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122087
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC79566.516664345917256No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC72425.931835494360579No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC66875.477241639158961No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC61515.038210456477757No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC50764.157690827033181No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC37683.086323687206664No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT23751.945334065051971No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC20091.6455478470271199No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT19331.5832971569454568No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT18691.5308755231924775No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT17751.453881248617789No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC15551.2736818825919223No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC14131.157371382702499No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT13981.1450850622916444No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC12841.0517090271691498No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC11180.9157404146223594No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT10500.8600424287598188No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC10380.8502133724311353No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8720.7142447598843448No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6640.5438744501871616No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6160.504558224872427No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5560.45541294322900877No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5430.4447647988729349No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4980.4079058376403712No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4950.40544857355820035No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4840.39643860525690694No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4670.3825141087912718No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4620.3784186686543203No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4610.37759958062693No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4330.3546651158600015No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4160.34074061939436634No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3790.3104343623809251No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3550.29077624972355776No Hit
CCTANGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3550.29077624972355776No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3460.283404457477045No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3120.2555554645457747No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3040.24900276032665233No Hit
CCTANGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2830.23180191175145595No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2670.21869650331321105No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2640.21623923923104016No Hit
CCTACGGGGGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2380.19494295051889227No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2350.19248568643672137No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2260.18511389419020863No Hit
CCTACGGGTGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2220.1818375420806474No Hit
CCTACGGGTGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT2110.17282757377935407No Hit
CCTACGGGGGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2080.17037030969718317No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2050.16791304561501225No Hit
CCTACGGGCGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2040.16709395758762194No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2010.16463669350545104No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1990.16299851745067043No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1830.14989310901242558No Hit
CCTACGGGTGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1810.14825493295764497No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1780.14579766887547405No Hit
CCTACGGGGGGCAGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1760.14415949282069343No Hit
CCTACGGGCGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1650.1351495245194001No Hit
CCTACGGGGGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1630.13351134846461948No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1480.12122502805376494No Hit
CCTACGGGCGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1460.11958685199898433No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1430.11712958791681342No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1430.11712958791681342No Hit
CCTACGGGAGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1420.1163104998894231No Hit
CCTACGGGGGGCAGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1410.11549141186203281No Hit
CCTACGGGTGGCTGCAGGCAGCAGGCAGCGCGCGGTACTGGCGAGTTCTC1400.11467232383464251No Hit
CCTACGGGGGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1390.11385323580725219No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1330.10893870764291037No Hit
CCTACGGGAGGCAGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1290.10566235553334917No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1280.10484326750595888No Hit
CCTACGGGGGGCTGCAGGCAGCAGGCAGCGCGCGGTACTGGCGAGTTCTC1260.10320509145117826No Hit
CCTACGGGGGGCAGCAGGCAGCAGGCAGCGCGCGGTACTGGCGAGTTCTC1250.10238600342378795No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1250.10238600342378795No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)