FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89236
Sequences flagged as poor quality0
Sequence length301
%GC53

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC20972.3499484512976827No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC18862.1134967950154646No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC17781.9924694069657984No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC14991.679815321170828No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC12931.4489667847057242No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG10381.1632076740329016No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG10351.159845802142633No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG10081.1295889551302165No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG9781.0959702362275316No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC9731.0903671164104174No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG8870.9939934555560536No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA8850.991752207629208No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG8800.9861490878120938No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT8580.9614953606167913No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA8440.9458066251288718No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA8000.896499170738267No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG7980.8942579228114214No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT7810.8752073154332333No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC7580.8494329642745081No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC7570.8483123403110853No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG7520.842709220493971No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG7200.8068492536644404No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT7170.8034873817741718No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG6990.7833161504325609No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA6990.7833161504325609No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6760.7575417992738357No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCG6380.714958088663768No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC6380.714958088663768No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6130.6869424895781971No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA6080.6813393697610829No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCG5770.6466000268949751No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5730.6421175310412838No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5420.607378188175176No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5410.606257564211753No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG5380.6028956923214845No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5370.6017750683580618No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC5070.5681563494553767No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCG5060.5670357254919539No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC4920.5513469900040343No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG4890.5479851181137658No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4690.525572638845309No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG4640.5199695190281949No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4560.5110045273208123No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4450.498677663723161No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCG4360.4885920480523555No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG4350.48747142408893274No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4320.48410955219866425No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG4320.48410955219866425No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4290.48074768030839565No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCG4240.4751445604912815No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCG4230.47402393652785874No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4210.471782688601013No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCG4160.4661795687838989No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG4100.4594558250033619No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG4050.45385270518624765No Hit
CCTANGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4000.4482495853691335No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3960.44376708951544225No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3880.4348020978080595No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG3860.4325608498812138No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC3710.41575149042987136No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCG3710.41575149042987136No Hit
CCTANGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3540.39670088305168316No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3530.3955802590882604No Hit
CCTANGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3260.36532341207584385No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCG3230.3619615401855753No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG3220.3608409162221525No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG3150.3529965484781927No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG3130.350755300551347No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2960.3317046931731588No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCG2880.32273970146577613No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2860.3204984535389305No Hit
CCTANGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2810.31489533372181633No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCG2780.31153346183154784No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2780.31153346183154784No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG2650.29696535030705096No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG2620.29360347841678247No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2590.290241606526514No Hit
CCTANGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2540.28463848670939984No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG2530.28351786274597696No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG2480.2779147429288628No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG2440.27343224707517144No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2430.2723116231117486No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2420.2711909991483258No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2360.26446725536778876No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2350.263346631404366No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG2330.26110538347752027No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2210.24765789591644627No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2180.24429602402617775No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2180.24429602402617775No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2150.24093415213590927No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2150.24093415213590927No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG2040.22860728853825812No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCG2030.22748666457483527No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2010.22524541664798958No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1990.22300416872114395No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG1990.22300416872114395No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1980.22188354475772112No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1970.22076292079429827No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1940.21740104890402975No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG1940.21740104890402975No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG1890.21179792908691558No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1840.20619480926980144No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1800.2017123134161101No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG1790.20059168945268727No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1790.20059168945268727No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1770.19835044152584158No Hit
CCTANGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1770.19835044152584158No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1760.19722981756241875No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1680.1882648258550361No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1640.18378233000134475No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1640.18378233000134475No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1630.18266170603792192No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCG1630.18266170603792192No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1610.18042045811107624No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG1590.17817921018423058No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG1510.16921421847684792No Hit
CCTANGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1490.16697297055000224No Hit
CCTANGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1480.1658523465865794No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1460.16361109865973372No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1460.16361109865973372No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1450.16249047469631092No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1430.16024922676946524No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1410.15800797884261958No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1350.15128423506208258No Hit
CCTANGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1340.15016361109865972No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1320.14792236317181406No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1300.1456811152449684No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG1290.14456049128154558No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1290.14456049128154558No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG1280.14343986731812272No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1280.14343986731812272No Hit
CCTANGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1260.14119861939127706No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCG1250.14007799542785423No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1230.13783674750100858No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1230.13783674750100858No Hit
CCTANGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1220.13671612353758572No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1210.1355954995741629No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG1190.13335425164731723No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1180.13223362768389438No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG1170.13111300372047158No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1160.12999237975704872No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG1150.1288717557936259No Hit
CCTANGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1140.12775113183020306No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1140.12775113183020306No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1120.12550988390335738No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1110.12438925993993456No Hit
CCTANGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1110.12438925993993456No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1100.12326863597651172No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCG1100.12326863597651172No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1050.11766551615939756No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1050.11766551615939756No Hit
CCTANGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1040.11654489219597472No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCG1040.11654489219597472No Hit
CCTANGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1010.11318302030570622No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCG990.11094177237886056No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG960.10757990048859206No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCG960.10757990048859206No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG960.10757990048859206No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC960.10757990048859206No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG930.10421802859832355No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCG930.10421802859832355No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT920.10309740463490072No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCG920.10309740463490072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)