FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151558
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA64224.237321685427361No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA45102.975758455508782No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA44062.9071378614127923No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG40772.6900592512437482No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA38892.5660143311471515No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA34442.272397366024888No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA29711.960305625569089No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG29611.9537074915213979No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG28331.869251375710949No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG26521.7498251494477362No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA24031.5855316116602225No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA23151.527468032040539No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG22701.497776428825928No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG18961.251006215442273No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG17421.1493949511078267No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG14870.9811425328916983No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG14550.960028503939086No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG14510.9573892503200095No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA13910.9178004460338616No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG11740.7746209371989601No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG11280.7442695205795801No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG10910.7198564246031223No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10340.6822470605312818No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9930.6551947109357473No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG9380.6189049736734451No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9180.6057087055780624No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG8700.5740376621491442No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8260.5450058723393024No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG7890.5205927763628446No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG7820.5159740825294606No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6590.43481703374285746No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG6020.397207669671017No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG5480.36157774581348395No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5400.3562992385753309No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5150.3398039034561026No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5050.3332057694084113No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5000.32990670238456565No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4780.31539080747964476No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4590.3028543527890312No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4510.2975758455508782No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4010.26458517531242165No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG3590.23687301231211816No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3560.23489357209781075No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3420.2256561844310429No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3300.21773842357381332No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3260.2150991699547368No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3240.21377954314519854No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3150.20784122250227635No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2950.19464495440689372No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2900.19134588738304806No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2800.18474775333535676No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2690.1774898058828963No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2660.17551036566858894No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2560.1689122316208976No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2550.1682524182161285No Hit
GANTACAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2350.15505615012074586No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2260.14911782947782368No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2260.14911782947782368No Hit
GANTANAAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2220.14647857585874713No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2190.14449913564443975No Hit
GACTACAAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA2130.14054025521582497No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2010.13262249435859538No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1900.12536454690613494No Hit
GACTACTAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1840.12140566647752014No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCACACGCTTTCGAGCCTCAA1790.11810659945367451No Hit
GACTANAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1780.11744678604890538No Hit
GANTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1780.11744678604890538No Hit
GACTACAGGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1750.11546734583459799No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1730.11414771902505971No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1690.11150846540598318No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1650.10886921178690666No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1650.10886921178690666No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1600.10557014476306101No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1580.10425051795352273No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1560.10293089114398449No Hit
GANTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1550.10227107773921534No Hit
GANTANAAGGGTATCTAATNCTGTTTGCTCCCCACGCTTTCGCACCTCAG1530.1009514509296771No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)