FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150296
Sequences flagged as poor quality0
Sequence length301
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC113597.557752701336031No Hit
CCTACGGGGGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC107937.181162505988184No Hit
CCTACGGGGGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC97246.469899398520253No Hit
CCTACGGGCGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC86735.770612657688828No Hit
CCTACGGGAGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC76495.089290466812157No Hit
CCTACGGGAGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC53533.5616383669558735No Hit
CCTACGGGTGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC30011.9967264597860221No Hit
CCTACGGGCGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC23911.5908607015489435No Hit
CCTANGGGTGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC19101.2708255708734764No Hit
CCTANGGGGGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC18471.2289082876457125No Hit
CCTANGGGGGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC16491.0971682546441688No Hit
CCTANGGGCGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC14880.9900463086176611No Hit
CCTANGGGAGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC12690.8443338478735296No Hit
CCTANGGGAGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC8730.5808537818704423No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA6480.4311491989141427No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5580.37126736573162294No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA5300.352637462074839No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5120.340661095438335No Hit
CCTANGGGTGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC5010.33334220471602705No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4970.33068078990791505No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA4720.3140469473572151No Hit
CCTACGGGTGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC4600.3060627029328791No Hit
CCTACGGGGGGCAGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC4320.28743279927609516No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA4090.2721296641294512No Hit
CCTANGGGCGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC4070.2707989567253952No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA4000.2661414808111992No Hit
CCTACGGGGGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC3790.25216905306861126No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3780.2515036993665833No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3740.2488422845584713No Hit
CCTACGGGCGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC3640.24218874753819128No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC3450.22954702719965933No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA3330.22156278277532335No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC3310.22023207537126738No Hit
CCTACGGGGGGCAGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC3300.21956672166923938No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC3210.2135785383509874No Hit
CCTACGGGGGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC3130.2082557087347634No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3100.2062596476286794No Hit
CCTACGGGTGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC3050.2029328791185394No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2860.19029115878000744No Hit
CCTACGGGAGGCAGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC2860.19029115878000744No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2850.18962580507797944No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2770.18430297546175547No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2680.1783147921435035No Hit
CCTACGGGCGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC2560.1703305477191675No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2450.16301165699685954No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA2450.16301165699685954No Hit
CCTACGGGAGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC2410.16035024218874755No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2380.15835418108266353No Hit
CCTACGGGAGGCAGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC2280.15170064406238357No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2280.15170064406238357No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2220.14770852185021557No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2070.13772821631979562No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1900.12641720338531964No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1880.12508649598126365No Hit
CCTACGGGTGGCTGCAGGCACATAGACAGATGCCAAGAGCGCATTGTCAG1860.12375578857720763No Hit
CCTACGGGGGGCAGCAGGCACATAGACAGATGCCAAGAGCGCATTGTCAG1830.12175972747112365No Hit
CCTACGGGGGGCAGCAGAGTGGTCAATGTTGAGGGAAGACATCCTGCAGA1820.12109437376909564No Hit
CCTACGGGGGGCTGCAGAGTGGTCAATGTTGAGGGAAGACATCCTGCAGA1820.12109437376909564No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1810.12042902006706765No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1780.11843295896098367No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1750.11643689785489966No Hit
CCTACGGGTGGCTGCAGTGAAGGTGTAACTCAGCCCTGTGTGATTGCAAT1730.11510619045084368No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1720.11444083674881567No Hit
CCTACGGGTGGCTGCAGAGTGGTCAATGTTGAGGGAAGACATCCTGCAGA1710.11377548304678767No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1650.10978336083461969No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1620.10778729972853568No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1610.1071219460265077No Hit
CCTACGGGGGGCTGCAGGCACATAGACAGATGCCAAGAGCGCATTGTCAG1600.1064565923244797No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1600.1064565923244797No Hit
CCTACGGGCGGCTGCAGGCACATAGACAGATGCCAAGAGCGCATTGTCAG1550.1031298238143397No Hit
CCTACGGGAGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC1540.10246447011231172No Hit
CCTACGGGTGGCTGCAGTTGCAACGGAGGAATTCTGTGGTGTTCCCGGTT1530.1017991164102837No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1530.1017991164102837No Hit
CCTACGGGGGGCAGCAGTGAAGGTGTAACTCAGCCCTGTGTGATTGCAAT1520.1011337627082557No Hit
CCTACGGGCGGCTGCAGTGAAGGTGTAACTCAGCCCTGTGTGATTGCAAT1520.1011337627082557No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)