FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81411
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC24723.036444706489295No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC22942.8178010342582698No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC19922.4468437926078788No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC19622.40999373549029No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC16322.004643107196816No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC12201.4985689894486003No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8681.0661949859355615No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8521.0465416221395143No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7130.8758030241613541No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC7070.8684330127378364No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6950.8536929898908009No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6720.825441279433983No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC6330.777536205181118No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6210.7627961823340826No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC5930.728402795691No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5020.6166242891009814No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC4900.601884266253946No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC4440.5453808453403103No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4090.502389112036457No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4060.4987041063246982No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3700.4544840377835919No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3300.40535062829347385No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3290.40412229305622094No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3240.3979806168699561No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3210.3942956111581973No Hit
CCTACGGGTGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA3190.39183894068369135No Hit
CCTACGGGGGGCAGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA2970.36481556546412647No Hit
CCTACGGGGGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA2930.35990222451511467No Hit
CCTACGGGTGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT2520.3095404797877437No Hit
CCTACGGGCGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA2520.3095404797877437No Hit
CCTACGGGGGGCAGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC2330.2862021102799376No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2300.28251710456817875No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2300.28251710456817875No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2250.276375428381914No Hit
CCTACGGGGGGCAGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT2230.2739187579074081No Hit
CCTACGGGTGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC2210.2714620874329022No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2210.2714620874329022No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2050.25180872363685497No Hit
CCTACGGGGGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT2010.24689538268784314No Hit
CCTACGGGAGGCAGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1980.24321037697608433No Hit
CCTACGGGGGGCAGCAGGACAGAGTGAGTGCAAGCAGGGGAAATGCCAGA1940.2382970360270725No Hit
CCTACGGGCGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1890.23215535984080776No Hit
CCTACGGGTGGCTGCAGGACAGAGTGAGTGCAAGCAGGGGAAATGCCAGA1890.23215535984080776No Hit
CCTACGGGGGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1850.22724201889179596No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1850.22724201889179596No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1830.22478534841729003No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1820.2235570131800371No Hit
CCTACGGGGGGCTGCAGGACAGAGTGAGTGCAAGCAGGGGAAATGCCAGA1810.22232867794278413No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1780.21864367223102527No Hit
CCTACGGGCGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1760.2161870017565194No Hit
CCTACGGGAGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1710.21004532557025463No Hit
CCTACGGGAGGCAGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1680.20636031985849576No Hit
CCTACGGGCGGCTGCAGGACAGAGTGAGTGCAAGCAGGGGAAATGCCAGA1660.2039036493839899No Hit
CCTANGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1610.19776197319772512No Hit
CCTACGGGAGGCAGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1580.19407696748596626No Hit
CCTANGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1440.17688027416442495No Hit
CCTACGGGTGGCTGCAGATGCTCCAGAGTTTGTGGATATGAAGAGTGTGG1420.17442360368991905No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1400.17196693321541315No Hit
CCTACGGGGGGCAGCAGATGCTCCAGAGTTTGTGGATATGAAGAGTGTGG1290.15845524560563068No Hit
CCTACGGGAGGCAGCAGGACAGAGTGAGTGCAAGCAGGGGAAATGCCAGA1270.15599857513112478No Hit
CCTANGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1250.1535419046566189No Hit
CCTACGGGAGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1240.1523135694193659No Hit
CCTANGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1230.15108523418211298No Hit
CCTANGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1170.14371522275859527No Hit
CCTANGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1150.14125855228408937No Hit
CCTACGGGCGGCTGCAGATGCTCCAGAGTTTGTGGATATGAAGAGTGTGG1150.14125855228408937No Hit
CCTACGGGGGGCTGCAGATGCTCCAGAGTTTGTGGATATGAAGAGTGTGG1120.13757354657233054No Hit
CCTACGGGTGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1110.13634521133507757No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1090.13388854086057167No Hit
CCTANGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1090.13388854086057167No Hit
CCTANGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1070.13143187038606577No Hit
CCTANGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1060.1302035351488128No Hit
CCTACGGGGGGCAGCAGCTCCTGTGGGAAGGAAGGAAACAGGAAATTTAC1030.12651852943705397No Hit
CCTACGGGGGGCAGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG980.1203768532507892No Hit
CCTACGGGTGGCTGCAGCTCCTGTGGGAAGGAAGGAAACAGGAAATTTAC970.11914851801353625No Hit
CCTACGGGAGGCTGCAGGACAGAGTGAGTGCAAGCAGGGGAAATGCCAGA970.11914851801353625No Hit
CCTACGGGAGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC960.1179201827762833No Hit
CCTACGGGAGGCAGCAGATGCTCCAGAGTTTGTGGATATGAAGAGTGTGG950.11669184753903036No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG940.11546351230177739No Hit
CCTACGGGTGGCTGCAGGGAGCTATGATCGCACTACTGCACTCCAGCCTG910.11177850659001855No Hit
CCTANGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC900.11055017135276561No Hit
CCTANGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC900.11055017135276561No Hit
CCTACGGGGGGCTGCAGGGAGCTATGATCGCACTACTGCACTCCAGCCTG890.10932183611551263No Hit
CCTACGGGTGGCAGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA890.10932183611551263No Hit
CCTACGGGTGGCTGCAGGTCCGAGTACTCGGGCCTCTTCCGCGGCGGCAG860.1056368304037538No Hit
CCTACGGGTGGCTGCAGAGGCAAGGCACACAAGTGTTCAAGTGATTAATC840.10318015992924788No Hit
CCTACGGGTGGCTGCAGTGAGCCATGATTGATCCACTGCACTCCAGCCTG830.10195182469199494No Hit
CCTACGGGCGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG820.100723489454742No Hit
CCTANGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC820.100723489454742No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)