FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81411
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA28763.5326921423394873No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA19362.378057019321713No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA18332.2515384898846595No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA16632.0427214995516576No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA15911.954281362469445No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA11941.4666322732800237No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA10281.2627286238960338No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA9921.2185085553549273No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA6990.8586063308398127No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6340.778764540418371No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA6320.776307869943865No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4940.6067976072029578No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4360.5355541634422867No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4280.5257274815442631No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4200.5159007996462395No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3810.4679957253933743No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3720.4569407082580978No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3560.43728734446205053No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3130.3844689292601737No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2950.36235889498962054No Hit
GACTACAAGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT2700.3316505140582968No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2590.3181388264485143No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2570.31568215597400845No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2490.30585547407598485No Hit
GACTACAAGGGTATCTAATCCATTTTGAGTTGATTTAGAATATGCAAAGG2310.2837454398054317No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2280.28006043409367287No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2260.27760376361916694No Hit
GACTACAAGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC2250.276375428381914No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2190.26900541695839625No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2080.2554937293486138No Hit
GACTACAAGGGTATCTAATCCATTCTGAGTTGGTTTTTGTACATGGTGTA2020.2481237179250961No Hit
GACTACAGGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1780.21864367223102527No Hit
GACTACACGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1710.21004532557025463No Hit
GACTACTAGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1680.20636031985849576No Hit
GACTACACGGGTATCTAATCCATTTTGAGTTGATTTAGAATATGCAAAGG1670.20513198462124285No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1620.19899030843497806No Hit
GACTACACGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1610.19776197319772512No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1570.19284863224871332No Hit
GACTACAGGGGTATCTAATCCATTCTGAGTTGGTTTTTGTACATGGTGTA1540.18916362653695445No Hit
GACTACAAGGGTATCTAATCCTCTGTCACATACCTCATCTCAACAGCCAA1530.18793529129970152No Hit
GACTACTCGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1520.18670695606244858No Hit
GACTACAGGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1520.18670695606244858No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1500.18425028558794265No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1470.18056527987618382No Hit
GACTACAGGGGTATCTAATCCATTTTGAGTTGATTTAGAATATGCAAAGG1460.17933694463893085No Hit
GACTACTAGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1450.17810860940167791No Hit
GACTACACGGGTATCTAATCCATTCTGAGTTGGTTTTTGTACATGGTGTA1370.16828192750365428No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1360.16705359226640135No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1340.16459692179189545No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1330.16336858655464248No Hit
GACTACCCGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1250.1535419046566189No Hit
GACTACTAGGGTATCTAATCCATTTTGAGTTGATTTAGAATATGCAAAGG1250.1535419046566189No Hit
GACTACTCGGGTATCTAATCCATTTTGAGTTGATTTAGAATATGCAAAGG1210.14862856370760708No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1180.14494355799584824No Hit
GACTACTAGGGTATCTAATCCATTCTGAGTTGGTTTTTGTACATGGTGTA1180.14494355799584824No Hit
GACTACAAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1170.14371522275859527No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1150.14125855228408937No Hit
GACTACCCGGGTATCTAATCCATTTTGAGTTGATTTAGAATATGCAAAGG1120.13757354657233054No Hit
GACTACTCGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1110.13634521133507757No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1110.13634521133507757No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1110.13634521133507757No Hit
GACTACTCGGGTATCTAATCCATTCTGAGTTGGTTTTTGTACATGGTGTA1090.13388854086057167No Hit
GACTACACGGGTATCTAATCCTCTGTCACATACCTCATCTCAACAGCCAA1070.13143187038606577No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1060.1302035351488128No Hit
GACTACAAGGGTATCTAATCCTCTTATTTCCTAAACAAAATAAAATGATC1030.12651852943705397No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1000.12283352372529512No Hit
GACTACCAGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1000.12283352372529512No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1000.12283352372529512No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG980.1203768532507892No Hit
GACTACCCGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC970.11914851801353625No Hit
GACTACTGGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT970.11914851801353625No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG960.1179201827762833No Hit
GACTACAAGGGTATCTAATCCCAGTCTTTATGAATTGGATATTTGCTAAT940.11546351230177739No Hit
GACTACCCGGGTATCTAATCCATTCTGAGTTGGTTTTTGTACATGGTGTA920.1130068418272715No Hit
GACTACAAGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC900.11055017135276561No Hit
GACTANAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA900.11055017135276561No Hit
GACTACAAGGGTATCTAATCCAATCATCTGATGAGGAAATTGAAGCTTAG860.1056368304037538No Hit
GACTACCAGGGTATCTAATCCATTTTGAGTTGATTTAGAATATGCAAAGG860.1056368304037538No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA850.10440849516650083No Hit
GACTACAGGGGTATCTAATCCTCTGTCACATACCTCATCTCAACAGCCAA840.10318015992924788No Hit
GACTACCAGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT830.10195182469199494No Hit
GACTACTGGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC830.10195182469199494No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)