FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12369404
Sequences flagged as poor quality0
Sequence length66-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT260650.09.9926405120
AGTGTAG359400.08.950245120
AGATCTC276150.08.910192120
GAGTGTA387100.08.619852120
AAGAGTG477350.08.498807120
GTGTAGA364450.08.348689120
AGAGTGT422850.08.30267120
ATCTCGG258800.08.301699120
AAAGAGT547350.08.245195120
TGTAGAT380550.08.18472120
GTAGATC370000.08.077479120
GTAGGGA730450.07.9530387120
TGGTCGC147700.07.8826120
TAGGGAA687400.07.8225207120
GATCTCG308700.07.659631120
TGTAGGG787200.07.5626645120
GAAAGAG660150.07.2272444120
GTGGTCG170450.07.217804120
GGGAAAG691150.07.1114774120
TCTCGGT236400.07.108186120
Produced by FastQC (version 0.11.5)