FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12369404
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC144950.050.707623120
GAGCACA203050.035.723286120
AGAGCAC262700.031.846556120
AAGAGCA379200.020.641388118-119
GAAGAGC445050.018.913683120
GGAAGAG555400.014.970556120
TCGGAAG628750.013.960465120
CGGAAGA654650.013.388501120
GATCGGA774300.012.357738120
CTAGATC45000.012.003614120
ATCGGAA756800.011.878766120
AGATCGG860850.010.912402118-119
CACGTCT50950.010.601818120
TAGATCG151600.010.2650585120
CACACGT53700.010.058895120
TTAGATC61150.09.885120
TCTAGAT20650.09.017403118-119
ACACGTC55200.08.782363118-119
CCTAGAT16650.08.677044118-119
AAGATCG399050.07.9605837120
Produced by FastQC (version 0.11.5)