FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12885826
Sequences flagged as poor quality0
Sequence length78-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTGT321200.09.392727120
GAGTGTA282150.09.05258120
AGTGTAG260600.08.970956120
TAGATCT187900.08.955602120
TGTAGGG594850.08.951375120
AAGAGTG364650.08.932779120
GTAGGGA548800.08.607399120
TAGGGAA523350.08.463282120
GTGTAGG661200.08.353071120
GTGTAGA267150.08.346075120
TCGTGTA745350.08.264701120
GTAGATC280300.08.104612120
ATCTCGG201000.08.07293120
AGATCTC204550.07.903443120
GGGAAAG530300.07.8537145120
AGGGAAA586300.07.8518577120
CGTGTAG697600.07.847829118-119
GTCGTGT796700.07.7772737120
TCTCGGT178700.07.6005917120
TGTAGAT303100.07.4354773120
Produced by FastQC (version 0.11.5)