FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12885826
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC125000.057.637554120
GAGCACA197200.040.10484120
AGAGCAC254400.031.611082120
AAGAGCA402750.020.196762118-119
GAAGAGC442300.018.190578118-119
GGAAGAG541700.016.508116120
TCGGAAG638750.013.781478120
CGGAAGA638100.013.43653118-119
GATCGGA766800.012.331979120
ATCGGAA756150.012.1953335120
AGATCGG858250.011.1288120
TAGATCG226050.010.884825118-119
CTAGATC54800.010.099593118-119
TTAGATC86150.08.92063116-117
CACACGT40350.08.802007120
GCTAGAT21350.08.317588120
CCTAGAT18700.08.286584108-109
CACGTCT47000.08.0963745120
GAGATCG360500.08.0574465120
AAGATCG321850.07.5859528118-119
Produced by FastQC (version 0.11.5)