FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13927413
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC247650.047.353195120
GAGCACA360450.033.049053120
AGAGCAC338550.032.153564120
AAGAGCA521700.022.262577120
GAAGAGC580650.019.260714120
CACGTCT108750.017.363209120
GGAAGAG667750.017.274252120
CGTCTGA120450.016.501705120
ACACGTC125400.015.691818120
ACGTCTG118700.015.461208120
CACACGT128650.015.140908120
CGGAAGA771550.014.882184118-119
TCGGAAG786450.014.478068118-119
ATCGGAA950350.012.876495120
GATCGGA1158550.012.312426120
TCTGAAC124650.011.852963120
TAGATCG296350.011.670244120
GCACACG163300.011.6041155118-119
AGATCGG1284050.011.114198120
CTAGATC75550.09.734658118-119
Produced by FastQC (version 0.11.5)