FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16155810
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC231650.096.86986120
GAGCACA375500.057.35946120
AGAGCAC521350.044.480217120
AAGAGCA763000.029.596422118-119
GAAGAGC869100.026.065756118-119
GGAAGAG1047400.021.53731116-117
CGGAAGA1216700.018.769558116-117
TCGGAAG1254300.017.955282114-115
ATCGGAA1440500.015.849979114-115
GATCGGA1494100.015.0994215112-113
AGATCGG1637250.013.903259112-113
CTAGATC78300.013.271964110-111
TAGATCG356500.013.056249112-113
TTAGATC100050.011.302064110-111
GAGATCG619250.011.110516110-111
AAGATCG522950.010.826695110-111
CAGATCG563700.010.086411110-111
ATAGATC171950.09.864245108-109
GCTAGAT27950.09.6868725110-111
CCTAGAT27500.09.41118112-113
Produced by FastQC (version 0.11.5)