FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15784402
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC199100.089.81519120
GAGCACA328550.054.33765120
AGAGCAC445850.040.23859118-119
AAGAGCA690450.026.102772120
GAAGAGC759100.023.677729116-117
GGAAGAG912800.019.715075116-117
CGGAAGA1064950.016.954319114-115
TCGGAAG1098950.016.365925114-115
TCCGATC94250.016.1247023
CCGATCT95050.015.8722794
CGATCTA25050.014.3922335
ATCGGAA1284100.014.150249112-113
GATCGGA1326500.013.659075120
CTTCCGA113450.013.4809441
AGATCGG1452850.012.50882110-111
TAGATCG395100.011.751688110-111
CTAGATC99650.010.830806120
CACACGT46800.010.416026118-119
ACACGTC52450.010.288749120
GAGATCG534150.09.930321110-111
Produced by FastQC (version 0.11.5)