FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12509078
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA742900.010.1570635120
GGTGGTC526550.08.536542120
TCGGTGG614550.08.391211120
CGTATCA282650.08.345225120
TGGTCGC448900.08.337614120
GTGGTCG485450.08.081725120
GGTCGCC444750.07.9418774120
GCCGTAT381300.07.811589120
GTATCAT297950.07.7955174120
CCGTATC354600.07.55131120
CGCCGTA404050.07.5206823120
CTCGGTG667600.07.463027120
CGGTGGT588050.07.449346120
TAGATCT837550.07.414297120
GATCTCG793550.07.2263603120
TCTCGGT700100.07.1985936118-119
ATCTCGG747050.07.152606120
ATCATTA279350.07.1513886120
TCGCCGT474550.07.062756120
GCGTCGG103300.07.048328120